P450Arom induction in isolated control endometrial cells by peritoneal fluid from women with endometriosis

Castro J, Jazmin Castro, M.J. Torres, Torres M, Marisa Torres, Hugo Sovino, Sovino H, Ariel Fuentes, Fuentes A, M Angélica Boric, M A Boric, Boric MA, Marı́a Cecilia Johnson, M. Cecilia Johnson, Johnson MC
Fertility and sterility · 2010 · vol. 94(7) , pp. 2521–2527 · doi:10.1016/j.fertnstert.2010.03.036 · PMID:20430378 · W2022333403
Lab / animal OA: bronze CC0 ⤵ 9 in-corpus citations
🔓 Open OA copy View on OpenAlex View on PubMed View at publisher
⚙ AI-generated summary by gemini-2.5-flash-lite, 2026-06-08 ⓘ

This study investigated the induction of P450Arom in isolated endometrial cells by peritoneal fluid from women with endometriosis.

One-sentence paraphrase of the abstract; not a substitute for reading it. No clinical advice. How this works

Abstract

OBJECTIVE: To study the effect of peritoneal fluid from women with (PF-E) and without (PF-C) endometriosis on P(450)Arom expression in endometrial cells. DESIGN: Experimental study. SETTING: University research unit. PATIENT(S): Forty women of reproductive age with (n = 22) or without (control; n = 18) endometriosis. INTERVENTION(S): Peritoneal fluid and eutopic endometrial samples were obtained during surgery from women with (n = 13 and 9, respectively) and without (n = 4 and 14, respectively) endometriosis. MAIN OUTCOME MEASURE(S): Expression study for P(450)Arom, steroid factor 1 (SF-1), chicken ovalbumin upstream transcription factor I (COUP-TFI), and COUP-TFII messenger RNA (reverse transcriptase-polymerase chain reacion) and/or protein (immunoblot) in isolated endometrial epithelial cells transfected or not with expression vector containing SF-1, COUP-TFI, or COUP-TFII complementary DNAs. RESULT(S): Basal messenger RNA and/or protein expression of P(450)Arom and SF-1 were augmented in endometriosis, and that of COUP-TF was diminished. In control cells, (Bu)(2)cAMP and PF-E increased P(450)Arom and SF-1 expression (but not COUP-TF expression) in a dose-dependent way, an effect not observed with PF-C, adsorbed PF-E, or 10(-5) M indomethacin. Transfected cells confirmed these results. Any treatments modified the studied molecules in endometriosis cells. CONCLUSION(S): These data indicate that molecules contained in PF-E favor an estrogenic microenvironment, suggesting a role in the etiopathogenesis of endometriosis enabling the survival, maintenance, and growth of endometrial implants in the ectopic locations.

My notes (saved in your browser only)

Condition tags

endometriosis

MeSH descriptors

Aromatase Ascitic Fluid Ascitic Fluid Endometriosis Endometrium Peritoneal Diseases Adult Adult Aromatase Aromatase Aromatase Ascitic Fluid Ascitic Fluid Case-Control Studies Case-Control Studies Cells, Cultured Cells, Cultured Cell Separation Cell Separation COUP Transcription Factors

Citation neighborhood (2-hop)

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. Outer rings show 2-hop neighbours — papers reached through the immediate citers/citees. [ collapse to 1-hop ]

References (57)

Cited by (9)

Source provenance

europepmc
last seen: 2026-10-16T06:09:50.462052+00:00
openalex
last seen: 2026-06-10T17:14:06.276822+00:00
pubmed
last seen: 2026-10-08T21:07:31.877465+00:00
License: CC0 · commercial use OK