Molecular detection of intrauterine microbial colonization in women with endometriosis

Khaleque N Khan, Khaleque Newaz Khan, Khan KN, Akira Fujishita, Fujishita A, Hiroshi Masumoto, Masumoto H, Muto H, Hideki Muto, Michio Kitajima, Kitajima M, Hideaki Masuzaki, Masuzaki H, Kitawaki J, Jo Kitawaki
European journal of obstetrics, gynecology, and reproductive biology · 2016 · vol. 199 , pp. 69–75 · doi:10.1016/j.ejogrb.2016.01.040 · PMID:26901400 · W2260592712
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This study aimed to identify microorganisms within the uterine cavity of women with endometriosis using molecular detection methods.

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Abstract

ObjectiveIncreased intrauterine microbial colonization by bacteria culture method and occurrence of endometritis have been reported in women with endometriosis. Here we investigated microbial colonization in intrauterine environment and cystic fluid of women with and without endometriosis by molecular approach.Study designThis is a case-controlled biological study with a total of 32 women each with and without endometriosis. Among them, 16 each in these two groups of women received treatment with gonadotropin-releasing hormone agonist (GnRHa). Pattern of microbial colonization in endometrial swabs and endometrioma/non-endometrioma cystic fluid was examined using broad-range polymerase-chain reaction (PCR) amplification of bacteria targeting 16S rRNA gene (rDNA). After quantification of index PCR product, 16S rDNA metagenome sequence analysis was done by Illumina Miseq system.ResultsA wide proportion (0.01-97.8%) of multiple bacteria was detected in both endometrial swabs and cystic fluid collected from women with and without endometriosis. 16S metagenome assay indicated that proportion of Lactobacillacae was significantly decreased (p<0.01) and of Streptococcaceae, Staphylococaceae, Enterobacteriaceae was significantly increased (p<0.05 for each) in GnRHa-treated women with endometriosis than in GnRHa-untreated women. While bacteria culture method failed to detect a single colony, 16S metagenome assay could detect significantly higher percentage of Streptococcaceae (p<0.01) and Staphylococaceae (p<0.05) in the cystic fluid derived from women with ovarian endometrioma comparing to that in cystic fluid collected from non-endometrioma cysts.ConclusionThese findings indicate the occurrence of sub-clinical infection in intrauterine environment and in the cystic fluid of ovarian endometrioma. Additional side effect of GnRHa treatment in promoting silent intrauterine and/or ovarian infection should be considered.

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Condition tags

mesh:D004716endometriosis

MeSH descriptors

Endometriosis Endometritis Lactobacillaceae Streptococcaceae Uterus Adult Case-Control Studies Endometriosis Endometriosis Endometritis Endometritis Female Gonadotropin-Releasing Hormone Gonadotropin-Releasing Hormone Humans Lactobacillaceae Leuprolide Leuprolide Metagenome Middle Aged

Citation neighborhood

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

References (32)

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