Fusobacterium nucleatum is not significantly present in eutopic endometrium from patients with minimal-mild and moderate-severe endometriosis

María Carmen Graciano-España, María Del Carmen Graciano-España, Kurt T. Barnhart, Kurt Barnhart, Marta Gonzáléz-Monfort, Marta Gonzalez-Monfort, Marta Arenas-Barrero, Richard S Legro, Tracey R Thomas, Tracey Thomas, Margaret Rush, Margaret A Rush, Felipe Vilella, Manuel Fernández-Sánchez, Manuel Fernández Sánchez, Carlos Simon, Carlos Simón, Inmaculada Moreno
Fertility and sterility · 2025 · vol. 124(5) , pp. 1071–1081 · doi:10.1016/j.fertnstert.2025.06.035 · PMID:40588151 · PMC12416407 · W4411873990
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⚙ AI-generated summary by claude@2026-06, 2026-06-06 ⓘ

This study investigated the presence of *Fusobacterium nucleatum* in endometrial tissue from women with and without endometriosis, finding no significant difference in its prevalence.

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Abstract

OBJECTIVE: To evaluate the presence of Fusobacterium spp. and Fusobacterium nucleatum in eutopic endometrial samples from women with endometriosis compared with controls, and assess their association with the disease. DESIGN: Retrospective case-control study. SUBJECTS: Ninety-two women (55 endometriosis cases and 37 controls) aged 18-44 years undergoing gynecologic endoscopy for endometriosis and/or benign conditions at University of Pennsylvania, Endomarker Study (PMID: 29524590). EXPOSURE: DNA extraction from eutopic endometrial samples using QIAamp DNA Microbiome and IndiSpin Pathogen Kits. Quantification of Fusobacterium spp. and F. nucleatum by quantitative PCR using genus- and species-specific primers. MAIN OUTCOME MEASURE(S): Relative abundance of Fusobacterium spp. and F. nucleatum in cases vs. controls, analyzed using the ΔCt method. RESULTS: No significant difference in Fusobacterium spp. or F. nucleatum abundance was observed between cases and controls (P = .258 for genus-specific primers, P = .738 for species-specific primers). Subgroup analysis by disease severity (minimal-mild: n = 42; moderate-severe: n = 13) also showed no significant differences (Fusobacterium spp.: P = .1465; F. nucleatum: P = .2936). CONCLUSION: Fusobacterium spp. is not differentially present in eutopic endometrium of women with endometriosis, regardless of disease severity according to revised American Society for Reproductive Medicine classification. This contrasts with prior findings in eutopic endometrium in patients with ovarian endometriosis, suggesting that Fusobacterium has limited diagnostic or prognostic value in endometriosis.

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Condition tags

endometriosis

MeSH descriptors

Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis

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Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

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