Human endometriosis-derived permanent cell line (FbEM-1): establishment and characterization

J Bouquet de Jolinière, Bouquet de Jolinière J, J. de Joliniere, Validire P, P Validire, M Canis, Michel Canis, Doussau M, M Doussau, Levardon M, M Levardon, Gogusev J, J Gogusev
Human Reproduction Update · 1997 · vol. 3(2) , pp. 117–123 · doi:10.1093/humupd/3.2.117 · PMID:9286736 · W2117625076
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A permanent human epithelial-like cell line, FbEM-1, was established from endometriosis implants and characterized for morphology, immunophenotype, receptor expression, and chromosomal aberrations.

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Abstract

A human epithelial-like cell line derived from peritoneal implants from a patient with gonadotrophin-releasing hormone (GnRH) agonist-resistant endometriosis graded as stage IVd according to the American Fertility Society classification was established in vitro. This cell line, designated FbEM-1, exhibited an epithelial-like morphology, grew in suspension and was immunoreactive for cytokeratins 8, 18, 19, vimentin and human leukocyte class I antigens. The cultured cells were negative for various haematopoietic cell markers, including the lymphoid cell antigens CD3, CD20 and CD45, von Willebrandt factor, carcinoembryonic antigen and the carcinoma antigen-125 (CA-125). In addition, the FbEM-1 cells were found to be moderately positive for periodic acid Schiff's (PAS) solution but were negative for alpha-naphthyl acetate esterase, peroxidase and chloroacetate esterase activities. Using specific antibodies against the progesterone, androgen and oestrogen receptors, approximately 40% and 5-10% of the cells immunostained for progesterone and androgen receptors respectively, while oestrogen receptors were not detected. On cytogenetic analysis using R-banding, these cells showed numerous chromosomal aberrations, including loss of one chromosome X, 4q+, 5q+, trisomy 7,8 and 10 and tetrasomy of chromosomes 17, 18, 19 and 20. These data show that the continuously growing FbEM-1 cell line established from endometriotic implants may be useful in achieving better understanding of the histogenesis of endometriosis.

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Condition tags

endometriosis

MeSH descriptors

Endometriosis Animals Animals Antigens, Surface Antigens, Surface Antigens, Surface Cell Division Cell Division Cell Line Cell Line Cell Transplantation Cell Transplantation Chromosome Aberrations Chromosome Aberrations Endometriosis Endometriosis Endometriosis Endometriosis Female Female

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