Circulating endometrial cells in peripheral blood

Vladimír Bobek, Vladimir Bobek, Katarína Kološtová, Kolostova K, Katarina Kolostova, Kucera E, Eduard Kucera, E Kučera
European journal of obstetrics, gynecology, and reproductive biology · 2014 · vol. 181 , pp. 267–274 · doi:10.1016/j.ejogrb.2014.07.037 · PMID:25195200 · W2092371672
article OA: closed CC0 ⤵ 15 in-corpus citations
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This paper investigates the presence and characteristics of circulating endometrial cells within peripheral blood samples.

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Abstract

ObjectivesEndometriosis is a common disorder amongst women of reproductive age. Despite extensive research, no reliable blood tests currently exist for the diagnosis of endometriosisStudy designWe report several new approaches enabling study of cell specific characteristic of endometrial cells, introducing enrichment and culturing of viable circulating endometrial cells (CECs) isolated from peripheral blood (PB) and peritoneal endometrial cells (PECs) from peritoneal washing (PW). Size-based enrichment method (MetaCell(®), Czech Republic) has been used for the filtration of PB and PW in patients with diagnosed endometriosis.ResultsThe PECs were found in the PW in all of the tested patients (n=17), but CECs) only in 23.5% (4/17) cases. Their endometrial origin has been proved by immunohistochemistry. PECs were successfully cultured in vitro directly on the separating membrane (9/17) exhibiting both endometrial cell phenotypes: stromal and glandular within the culture. CECs were successfully cultured in the two of the four positive cases, but in none of them confluence has been reached. The occurrence in CECs in PB is clear and very specific evidence of an active endometrial disease.ConclusionsWe demonstrated efficient, quick and user friendly endometrial cells capture platform based on a cell size. Furthermore, we demonstrated an ability to culture the captured cells, a critical requirement for post-isolation cellular analysis directed to better understanding of endometriosis pathogenesis.

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Condition tags

endometriosis

MeSH descriptors

Ascitic Fluid Endometriosis Endometriosis Endometrium Adult Ascitic Fluid Cell Culture Techniques Cells, Cultured Cells, Cultured Cells, Cultured Cell Separation Endometriosis Female Humans Immunohistochemistry Peritoneal Lavage

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Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

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License: CC0 · commercial use OK