Non-invasive diagnosis of endometriosis based on a combined analysis of six plasma biomarkers

A Mihalyi, Mihalyi A, Attila Mihalyi, Olivier Gevaert, O Gevaert, Cleophas Kyama, C M Kyama, Simsa P, Péter Simsa, P Simsa, N Pochet, Nathalie Pochet, F De Smet, Frank De Smet, Bart De Moor, De Moor B, B. De Moor, Christel Meuleman, C Meuleman, Meuleman C, Jaak Billen, J Billen, Billen J, Norbert Blanckaert, Blanckaert N, N Blanckaert, Vodolazkaia A, A Vodolazkaia, Alexandra Vodolazkaia, V Fulop, Vilmos Fülöp, Fulop V, D'Hooghe TM, Thomas D’Hooghe, T M D'Hooghe
Human Reproduction · 2009 · vol. 25(3) , pp. 654–664 · doi:10.1093/humrep/dep425 · PMID:20007161 · W2143886331
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A combined analysis of six plasma biomarkers (IL-6, IL-8, TNF-α, hsCRP, CA-125, CA-19-9) using advanced statistical methods accurately diagnosed minimal-mild and moderate-severe endometriosis.

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Abstract

BACKGROUND: Lack of a non-invasive diagnostic test contributes to the long delay between onset of symptoms and diagnosis of endometriosis. The aim of this study was to evaluate the combined performance of six potential plasma biomarkers in the diagnosis of endometriosis. METHODS: This case-control study was conducted in 294 infertile women, consisting of 93 women with a normal pelvis and 201 women with endometriosis. We measured plasma concentrations of interleukin (IL)-6, IL-8, tumour necrosis factor-alpha, high-sensitivity C-reactive protein (hsCRP), and cancer antigens CA-125 and CA-19-9. Analyses were done using the Kruskal-Wallis test, Mann-Whitney test, receiver operator characteristic, stepwise logistic regression and least squares support vector machines (LSSVM). RESULTS: Plasma levels of IL-6, IL-8 and CA-125 were increased in all women with endometriosis and in those with minimal-mild endometriosis, compared with controls. In women with moderate-severe endometriosis, plasma levels of IL-6, IL-8 and CA-125, but also of hsCRP, were significantly higher than in controls. Using stepwise logistic regression, moderate-severe endometriosis was diagnosed with a sensitivity of 100% (specificity 84%) and minimal-mild endometriosis was detected with a sensitivity of 87% (specificity 71%) during the secretory phase. Using LSSVM analysis, minimal-mild endometriosis was diagnosed with a sensitivity of 94% (specificity 61%) during the secretory phase and with a sensitivity of 92% (specificity 63%) during the menstrual phase. CONCLUSIONS: Advanced statistical analysis of a panel of six selected plasma biomarkers on samples obtained during the secretory phase or during menstruation allows the diagnosis of both minimal-mild and moderate-severe endometriosis with high sensitivity and clinically acceptable specificity.

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Condition tags

endometriosis

MeSH descriptors

Biomarkers Endometriosis Biomarkers Biomarkers CA-125 Antigen CA-125 Antigen CA-125 Antigen CA-19-9 Antigen CA-19-9 Antigen CA-19-9 Antigen Case-Control Studies Case-Control Studies C-Reactive Protein C-Reactive Protein C-Reactive Protein Endometriosis Endometriosis Endometriosis Female Female

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Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. Outer rings show 2-hop neighbours — papers reached through the immediate citers/citees. [ collapse to 1-hop ]

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