O soro de mulheres com endometriose altera os níveis de citocinas produzidas pelas células estromais e endoteliais uterinas cocultivadas em sistema 3D.

Caroline Borgato Guedes
In: Universidade de São Paulo · 2017 · doi:10.11606/d.42.2017.tde-10052017-134839 · W2658193161
dissertation OA: gold CC0
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⚙ AI-generated summary by claude@2026-06, 2026-06-07 ⓘ

Sera from women with endometriosis, especially those not using contraception, altered cytokine production by cocultured uterine stromal and endothelial cells, with contraceptive use correlating with increased IL6 and IL8.

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⚙ AI-generated deep summary by claude@2026-06, 2026-06-07 ⓘ

This thesis studied whether serum from women with endometriosis can alter cytokine profiles produced by a partially reconstituted human endometrium in a 3D co-culture system comprising selected uterine stromal and endothelial cells. Endometrial biopsies and sera were collected from 15 women without endometriosis and 15 women with endometriosis (10 using contraceptives and 5 untreated post-surgery), after which the cells were cultured in 3D and exposed to either healthy serum or endometriosis serum for 24–48 hours, with cytokines quantified by cytometric bead array; the reconstituted tissue was also characterized by immunofluorescence. The model produced a cytokine response featuring IL-2, IL-10, IL-6, IFN-γ, and TNF-α with an overall inflammatory tendency, and sera from treated patients tended toward a less-exacerbating inflammatory cytokine pattern (including IL-4, IL-6, and IL-8). A major caveat explicitly noted is the limited group sizes and stratification (n=3–8 per condition), which constrains resolution of effects by treatment status. This paper is centrally about endometriosis — it investigates how endometriosis patient serum reshapes cytokine production in a 3D uterine stromal–endothelial co-culture model relevant to infertility.

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Abstract

\n A endometriose é caracterizada pela presença de tecido endometrial fora do útero. No estudo, utilizou-se um sistema de co-cultivo 3D contendo células do estroma e endotélio endometrial. O sistema foi exposto ao soro de mulheres saudáveis ou com endometriose, que fazem ou não o uso de anticoncepcional. Posteriormente, foi avaliada a resposta do sistema no que diz respeito ao perfil de citocinas. Nossos resultados mostraram que o perfil sérico das mulheres com endometriose sem anticoncepcional apresentaram elevados níveis séricos de IL2 e IL10 com relação às saudáveis. Enquanto que as mulheres com endometriose, que fazem uso de anticoncepcional, apresentaram altos níveis de IL6 e IL8. O perfil de citocinas encontrado no homogenato das mulheres com endometriose induziu a produção exacerbada de IL2 e IL10 além de IFN-γ, TNF-α, IL6. Quando as células eram incubadas com soro de mulheres com endometriose que fazem tratamento, os níveis de IL6 e IL8 aumentaram. Os achados sugerem que o uso de co-cultivos 3D de células estromais e endoteliais endometriais é um bom modelo para novos estudos.\n

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