Isolation and culture of human endometrial cells in a three-dimensional culture system
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This study developed a three-dimensional culture system that successfully isolated and maintained human endometrial epithelial and stromal cells, mimicking the native endometrial structure.
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Abstract
A cell culture system was established in which endometrial stromal cells were embedded in a collagen matrix and separated from the endometrial epithelium by basement membrane material (Matrigel). The epithelium, seeded on top of the collagen matrix, grew in a monolayer. The cultures were evaluated by light microscopy and transmission and scanning electron microscopy. Light and transmission electron microscopy indicated a polarized columnar epithelium in monolayer with basally positioned nuclei. Scanning electron microscopy revealed a confluent epithelium with an abundance of microvilli and cilia, as well as pinopodes on the apical surface. Immunohistochemical staining for cytokeratin confirmed the epithelial origin of the surface cells, and staining for human collagen IV demonstrated its presence underneath the epithelial cells. This culture system represents a three-dimensional system that imitates the normal endometrium.
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Cited by (16)
- Three-Dimensional Organoid-like Co-Culture of Human Endometrial Endothelial and Stromal Cells to Study Endometriosis-Associated Responses 2026
- Computational modeling of hormone- and cytokine-dependent proliferation of endometrial cells in 3D co-culture 2025
- WERF Endometriosis Phenome and Biobanking Harmonisation Project for Experimental Models in Endometriosis Research (EPHect-EM-Organoids): endometrial organoids as an emerging technology for endometriosis research 2025
- Advances in the use of organoids in endometrial diseases 2024
- A Revised Stem Cell Theory for the Pathogenesis of Endometriosis 2022
- Building a stem cell-based primate uterus 2021
- Organoid co-culture model of the cycling human endometrium in a fully-defined synthetic extracellular matrix reveals epithelial-stromal crosstalk 2021
- Menstruation: science and society 2020
- Physiomimetic Models of Adenomyosis 2020
- Long-term, hormone-responsive organoid cultures of human endometrium in a chemically defined medium 2017
- Long-term, hormone-responsive organoid cultures of human endometrium in a chemically defined medium 2017
- O soro de mulheres com endometriose altera os níveis de citocinas produzidas pelas células estromais e endoteliais uterinas cocultivadas em sistema 3D. 2017
- Endometriotic Epithelial Cell Response to Macrophage-Secreted Factors is Dependent on Extracellular Matrix Context 2014
- A tissue-engineered human endometrial stroma that responds to cues for secretory differentiation, decidualization, and menstruation 2012
- Endometrial Organoid Culture 2012
- Endometrial paradigms 2008
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- last seen: 2026-06-10T17:14:06.276822+00:00
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