Gonadotropin-releasing hormone agonist induces apoptosis and reduces cell proliferation in eutopic endometrial cultures from women with endometriosis

Gabriela F Meresman, Gabriela Meresman, Mariela Bilotas, Buquet RA, Ricardo A Buquet, Ricardo Buquet, Rosa Inés Barañao, Barañao RI, Rosa I Barañao, Sueldo C, Carlos Sueldo, Tesone M, Marta Tesone
Fertility and sterility · 2003 · vol. 80 , pp. 702–707 · doi:10.1016/s0015-0282(03)00769-6 · PMID:14505742 · W2046062301
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This study investigated the effects of gonadotropin-releasing hormone agonist on eutopic endometrial cultures from women with endometriosis, observing induction of apoptosis and reduced cell proliferation.

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Abstract

OBJECTIVE: There is growing evidence that suggests a direct action of gonadotropin-releasing hormone agonist (GnRH-a) on endometrial growth. Consequently, our purpose was to evaluate the effect of GnRH-a on in vitro eutopic endometrial cell growth and apoptosis. DESIGN: Prospective study. SETTING: Research institute and clinical fertility center. PATIENT(S): Sixteen women with untreated endometriosis and 14 controls. INTERVENTION(S): Biopsy specimens of eutopic endometrium were obtained from all subjects. Apoptosis and cell proliferation were examined in epithelial endometrial cell cultures after incubation with leuprolide acetate (LA), antide, and a combination of both. MAIN OUTCOME MEASURE(S): The percentage of apoptotic cells was evaluated by the acridine orange-ethidium bromide technique; cell proliferation was assessed by (3)H-thymidine incorporation. RESULT(S): Leuprolide acetate (LA) (100 ng/mL) enhanced apoptosis in endometrial cultures from patients with endometriosis and controls, and this effect was reversed by antide 10(-7)M. Cell proliferation was down-regulated by LA at 1, 10, and 100 ng/mL in cultures from women without and with endometriosis. The addition of antide 10(-7)M reversed this inhibition. CONCLUSION(S): GnRH-a appears to have a direct effect by enhancing the apoptotic index and decreasing the cell proliferation in endometrial cells.

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Condition tags

endometriosis

MeSH descriptors

Apoptosis Endometriosis Fertility Agents, Female Gonadotropin-Releasing Hormone Leuprolide Acridine Orange Acridine Orange Acridine Orange Apoptosis Apoptosis Apoptosis Biopsy Biopsy Cell Division Cell Division Cell Division Cell Division Endometriosis Endometriosis Epithelial Cells

Citation neighborhood (2-hop)

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. Outer rings show 2-hop neighbours — papers reached through the immediate citers/citees. [ collapse to 1-hop ]

References (29)

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