Fluorescence diagnosis of endometriosis on the chorioallantoic membrane using 5-aminolaevulinic acid

E Malik, Malik E, Eduard Malik, A. Meyhöfer-Malik, Meyhöfer-Malik A, Berg C, C. Berg, W Böhm, Böhm W, Karin Kunzi‐Rapp, K Kunzi-Rapp, Kunzi-Rapp K, K. Diedrich, Diedrich K, Rück A, A. Rück
Human Reproduction · 2000 · vol. 15(3) , pp. 584–588 · doi:10.1093/humrep/15.3.584 · PMID:10686200 · W2000969159
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This study demonstrates that 5-aminolaevulinic acid selectively accumulates in ectopic endometrium grafted onto the chorioallantoic membrane, allowing for fluorescence diagnosis differentiated from normal tissues.

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Abstract

The chorioallantoic membrane (CAM) is a useful model for the fluorescence diagnosis of experimentally induced endometriosis. In our experimental setup 75.7% of the histologically examined tissue preparations were viable and only 24.3% showed signs of necrosis on the CAM after various periods of incubation. Best results were obtained when grafting to the CAM was performed between days 7 and 9 and when implants were left on the CAM for 3-5 days (P < 0.05). We were able to demonstrate that 5-aminolaevulinic acid (ALA) is stored selectively in ectopic endometrium. The subsequent fluorescence of the endometrium shows a rapid increase that reaches a peak after 10-14 h which can be clearly differentiated from the weaker fluorescence of grafted normal peritoneum and fimbriae (P < 0.01).

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Condition tags

endometriosis

MeSH descriptors

Allantois Aminolevulinic Acid Aminolevulinic Acid Endometriosis Adult Adult Allantois Allantois Allantois Animals Animals Chick Embryo Chick Embryo Endometriosis Endometriosis Endometrium Endometrium Endometrium Endometrium Endometrium

Citation neighborhood (2-hop)

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. Outer rings show 2-hop neighbours — papers reached through the immediate citers/citees. [ collapse to 1-hop ]

References (27)

Cited by (12)

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