Fluorescence diagnosis of endometriosis on the chorioallantoic membrane using 5-aminolaevulinic acid

Eduard Malik, E Malik, A Meyhöfer-Malik, C Berg, W Böhm, Karin Kunzi‐Rapp, K Kunzi-Rapp, K Diedrich, A Rück
Human Reproduction · 2000 · vol. 15(3) , pp. 584–588 · doi:10.1093/humrep/15.3.584 · PMID:10686200 · W2000969159
article OA: closed CC0 ⤵ 12 in-corpus citations
View on OpenAlex View on PubMed View at publisher
⚙ AI-generated summary by claude@2026-06, 2026-06-10 ⓘ

This study demonstrates that 5-aminolaevulinic acid selectively accumulates in ectopic endometrium grafted onto the chorioallantoic membrane, allowing for fluorescence diagnosis differentiated from normal tissues.

One-sentence paraphrase of the abstract; not a substitute for reading it. No clinical advice. How this works

Abstract

The chorioallantoic membrane (CAM) is a useful model for the fluorescence diagnosis of experimentally induced endometriosis. In our experimental setup 75.7% of the histologically examined tissue preparations were viable and only 24.3% showed signs of necrosis on the CAM after various periods of incubation. Best results were obtained when grafting to the CAM was performed between days 7 and 9 and when implants were left on the CAM for 3-5 days (P < 0.05). We were able to demonstrate that 5-aminolaevulinic acid (ALA) is stored selectively in ectopic endometrium. The subsequent fluorescence of the endometrium shows a rapid increase that reaches a peak after 10-14 h which can be clearly differentiated from the weaker fluorescence of grafted normal peritoneum and fimbriae (P < 0.01).

My notes (saved in your browser only)

Condition tags

mesh:D004715endometriosis

MeSH descriptors

Allantois Aminolevulinic Acid Endometriosis Adult Allantois Allantois Animals Chick Embryo Endometriosis Endometrium Endometrium Endometrium Endometrium Erythrocytes Erythrocytes Female Fluorescence Humans Neovascularization, Physiologic Time Factors

Citation neighborhood

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

References (27)

Cited by (12)

Source provenance

europepmc
last seen: 2026-06-04T01:30:01.192114+00:00
openalex
last seen: 2026-06-10T17:14:06.276822+00:00
pubmed
last seen: 2026-05-13T22:13:41.710148+00:00
License: CC0 · commercial use OK