Quantitative analysis of estrogen receptor alpha and beta messenger ribonucleic acid levels in normal endometrium and ovarian endometriotic cysts using a real-time reverse transcription-polymerase chain reaction assay

Matsuzaki S, S Matsuzaki, Sachiko Matsuzaki, Uehara S, Shigeki Uehara, S Uehara, Murakami T, T Murakami, Takashi Murakami, Junko Fujiwara, J Fujiwara, Fujiwara J, Tadao Funato, T Funato, Funato T, Kunihiro Okamura, Okamura K, K. Okamura
Fertility and sterility · 2000 · vol. 74(4) , pp. 753–759 · doi:10.1016/s0015-0282(00)00712-3 · PMID:11020519 · W2063284433
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This study quantified estrogen receptor alpha and beta mRNA levels in normal endometrium and ovarian endometriotic cysts using real-time RT-PCR.

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Abstract

OBJECTIVE: To quantify messenger RNA (mRNA) levels of the two estrogen receptor isoforms, estrogen receptor-alpha (ER-alpha) and estrogen receptor-beta (ER-beta) in the eutopic endometrium and ovarian endometriotic cysts. DESIGN: Prospective study. SETTING: University hospital. PATIENT(S): Patients with endometriosis and patients with uterine leiomyoma or carcinoma in situ. INTERVENTION(S): Gonadotropin-releasing hormone agonist (GnRH-a)-treated (n = 12) or untreated (n = 24) endometriotic cysts were obtained from 36 patients during laparoscopic cystectomy. Eutopic endometrium tissues were obtained from 24 patients during or immediately after surgery. MAIN OUTCOME MEASURE(S): ER-alpha and ER-beta mRNA levels, using a real-time reverse transcription (RT)-polymerase chain reaction (PCR) assay, TaqMan RT-PCR. RESULT(S): Eutopic endometrium and ovarian endometriotic cysts showed predominantly higher levels of ER-alpha mRNA than ER-beta mRNA. Although ER-alpha and ER-beta mRNA levels in the eutopic endometrium were affected by a cyclic change in ovarian hormones, ovarian endometriotic cysts were less affected. Moreover, a long-term hypoestrogenic state induced by GnRH-a especially decreased ER-alpha mRNA levels in endometriotic cysts. Consequently, the relative ratios of ER-alpha to ER-beta mRNA levels in both GnRH-a-treated and untreated endometriotic cysts were significantly lower than those in the eutopic endometrium. CONCLUSION(S): The results suggest that the principal and regulatory effects of estrogens may be mediated mainly via ER-alpha rather than ER-beta in both the eutopic endometrium and endometriotic cysts.

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Condition tags

endometriosis

MeSH descriptors

Endometriosis Endometrium Ovary Receptors, Estrogen RNA, Messenger Adult Adult Delayed-Action Preparations Delayed-Action Preparations Endometriosis Endometriosis Endometriosis Endometrium Endometrium Estrogen Receptor alpha Estrogen Receptor alpha Estrogen Receptor beta Estrogen Receptor beta Female Female

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References (28)

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