Quantitative analysis of estrogen receptor alpha and beta messenger ribonucleic acid levels in normal endometrium and ovarian endometriotic cysts using a real-time reverse transcription-polymerase chain reaction assay

Matsuzaki S, S Matsuzaki, Sachiko Matsuzaki, Uehara S, Shigeki Uehara, S Uehara, Murakami T, T Murakami, Takashi Murakami, Junko Fujiwara, J Fujiwara, Fujiwara J, Tadao Funato, T Funato, Funato T, Kunihiro Okamura, Okamura K, K Okamura
Fertility and Sterility · 2000 · vol. 74(4) , pp. 753–759 · doi:10.1016/s0015-0282(00)00712-3 · PMID:11020519 · W2063284433
article OA: closed CC0 ⤵ 18 in-corpus citations
View on OpenAlex View on PubMed View at publisher
⚙ AI-generated summary by claude@2026-06, 2026-06-08 ⓘ

This study quantified estrogen receptor alpha and beta mRNA levels in normal endometrium and ovarian endometriotic cysts using real-time RT-PCR.

One-sentence paraphrase of the abstract; not a substitute for reading it. No clinical advice. How this works

Abstract

ObjectiveTo quantify messenger RNA (mRNA) levels of the two estrogen receptor isoforms, estrogen receptor-alpha (ER-alpha) and estrogen receptor-beta (ER-beta) in the eutopic endometrium and ovarian endometriotic cysts.DesignProspective study.SettingUniversity hospital.Patient(s)Patients with endometriosis and patients with uterine leiomyoma or carcinoma in situ.Intervention(s)Gonadotropin-releasing hormone agonist (GnRH-a)-treated (n = 12) or untreated (n = 24) endometriotic cysts were obtained from 36 patients during laparoscopic cystectomy. Eutopic endometrium tissues were obtained from 24 patients during or immediately after surgery.Main outcome measure(s)ER-alpha and ER-beta mRNA levels, using a real-time reverse transcription (RT)-polymerase chain reaction (PCR) assay, TaqMan RT-PCR.Result(s)Eutopic endometrium and ovarian endometriotic cysts showed predominantly higher levels of ER-alpha mRNA than ER-beta mRNA. Although ER-alpha and ER-beta mRNA levels in the eutopic endometrium were affected by a cyclic change in ovarian hormones, ovarian endometriotic cysts were less affected. Moreover, a long-term hypoestrogenic state induced by GnRH-a especially decreased ER-alpha mRNA levels in endometriotic cysts. Consequently, the relative ratios of ER-alpha to ER-beta mRNA levels in both GnRH-a-treated and untreated endometriotic cysts were significantly lower than those in the eutopic endometrium.Conclusion(s)The results suggest that the principal and regulatory effects of estrogens may be mediated mainly via ER-alpha rather than ER-beta in both the eutopic endometrium and endometriotic cysts.

My notes (saved in your browser only)

Condition tags

endometriosis

MeSH descriptors

Endometriosis Endometrium Ovary Receptors, Estrogen RNA, Messenger Adult Delayed-Action Preparations Endometriosis Endometriosis Endometrium Estrogen Receptor alpha Estrogen Receptor beta Female Humans Leuprolide Leuprolide Leuprolide Ovary Prospective Studies Receptors, Estrogen

Citation neighborhood

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

References (28)

Cited by (18)

Source provenance

europepmc
last seen: 2026-07-27T06:15:28.040536+00:00
openalex
last seen: 2026-06-10T17:14:06.276822+00:00
pubmed
last seen: 2026-05-13T22:13:36.046895+00:00
License: CC0 · commercial use OK