Disturbed balance between phase I and II metabolizing enzymes in ovarian endometriosis: a source of excessive hydroxy-estrogens and ROS?

Hevir N, Neli Hevir, Martina Ribič-Pucelj, Ribič-Pucelj M, Tea Lanišnik Rižner
Molecular and cellular endocrinology · 2012 · vol. 367(1-2) , pp. 74–84 · doi:10.1016/j.mce.2012.12.019 · PMID:23277161 · W2000224370
Other OA: closed CC0 ⤵ 23 in-corpus citations
View on OpenAlex View on PubMed View at publisher
⚙ AI-generated summary by gemini-2.5-flash-lite, 2026-06-08 ⓘ

This study investigated the balance of phase I and II metabolizing enzymes in ovarian endometriosis, hypothesizing a potential link to excessive hydroxy-estrogens and reactive oxygen species (ROS).

One-sentence paraphrase of the abstract; not a substitute for reading it. No clinical advice. How this works

Abstract

Oxidative metabolism of estrogens was studied in 31 ovarian endometriosis and 29 normal endometrium samples, by qPCR. Expression was monitored for genes encoding five estrogen hydroxylating, five hydroxy (OH)-estrogen conjugating, and three estrogen quinone detoxifying enzymes. CYP1B1, COMT, NQO1, and GSTP1 protein levels were determined using Western blotting and immunohistochemistry staining. Increased expression of CYP1A1, CYP3A7 and COMT, and higher levels of MB-COMT were seen in endometriosis, as compared to normal endometrium. Expression of CYP1B1, CYP3A5, SULT1A1 and NQO2 was unchanged, with comparable CYP1B1 protein levels. Expression of SULT1E1, SULT2B1, UGT2B7, NQO1, and GSTP1 was decreased. Three NQO1 isoforms were detected; NQO1c appears to be endometriosis-specific. Our data indicate a disturbed balance between phase I and II metabolizing enzymes in endometriosis, potentially leading to excessive OH-estrogen and altered ROS formation, and stimulation of proliferation of ectopic endometrium. This is the first report on disturbed expression of estrogen oxidative metabolism genes in ovarian endometriosis.

My notes (saved in your browser only)

Condition tags

endometriosis

MeSH descriptors

Endometriosis Endometriosis Estrogens Metabolic Detoxication, Phase I Metabolic Detoxication, Phase II Ovary Reactive Oxygen Species Adult Adult Aryl Hydrocarbon Hydroxylases Aryl Hydrocarbon Hydroxylases Aryl Hydrocarbon Hydroxylases Aryl Hydrocarbon Hydroxylases Blotting, Western Blotting, Western Case-Control Studies Case-Control Studies Catechol O-Methyltransferase Catechol O-Methyltransferase Catechol O-Methyltransferase

Citation neighborhood (2-hop)

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. Outer rings show 2-hop neighbours — papers reached through the immediate citers/citees. [ collapse to 1-hop ]

References (53)

Cited by (23)

Source provenance

europepmc
last seen: 2026-10-26T06:15:05.866214+00:00
openalex
last seen: 2026-06-10T17:14:06.276822+00:00
pubmed
last seen: 2026-10-08T21:11:19.299510+00:00
unpaywall
last seen: 2026-10-26T06:31:53.434635+00:00
License: CC0 · commercial use OK