Targeting of syndecan-1 by micro-ribonucleic acid miR-10b modulates invasiveness of endometriotic cells via dysregulation of the proteolytic milieu and interleukin-6 secretion

Cornelia Schneider, Schneider C, Nadja Kässens, Kässens N, Burkhard Greve, Greve B, Hebatallah Hassan, Andreas N. Schüring, Starzinski-Powitz A, Anna Starzinski‐Powitz, Anna Starzinski-Powitz, Kiesel L, Ludwig Kiesel, Seidler DG, Daniela G. Seidler, Martin Götte
Fertility and sterility · 2012 · vol. 99(3) , pp. 871–881.e1 · doi:10.1016/j.fertnstert.2012.10.051 · PMID:23206733 · W2007362663
article OA: bronze CC0 ⤵ 25 in-corpus citations
🔓 Open OA copy View on OpenAlex View on PubMed View at publisher
⚙ AI-generated summary by claude@2026-06, 2026-06-08 ⓘ

Micro-ribonucleic acid miR-10b targets syndecan-1 to influence endometriotic cell invasiveness by altering the proteolytic environment and interleukin-6 secretion.

One-sentence paraphrase of the abstract; not a substitute for reading it. No clinical advice. How this works

Abstract

ObjectiveTo study the function of syndecan-1 (SDC1) and its potential regulator miR-10b in endometriosis.DesignExperimental laboratory study.SettingUniversity medical center.Patient(s)Not applicable.Intervention(s)The human endometriotic cell line 12Z was transiently transfected with SDC1 small interfering RNA or miR-10b precursors and investigated for changes in cell behavior and gene expression. 12Z and primary eutopic endometrial stroma cells of two American Society for Reproductive Medicine class III endometriosis patients were transfected with miR-10b precursors to investigate posttranscriptional regulation of SDC1.Main outcome measure(s)Quantitative polymerase chain reaction, Western blotting, flow cytometry, 3' untranslated region luciferase assays, and zymography were used to measure miR-10b-dependent targeting of SDC1 and SDC1-dependent expression changes of proteases and interleukin-6. Altered cell behavior was monitored by Matrigel invasion assays, cell viability assays, and mitogen-activated protein kinase activation blots.Result(s)Knockdown of SDC1 inhibited Matrigel invasiveness by >60% but did not affect cell viability. Interleukin-6 secretion, matrix metalloproteinase-9 expression, and matrix metalloproteinase-2 activity were reduced, whereas plasminogen activator inhibitor-1 protein expression was up-regulated. miR-10b overexpression significantly down-regulated SDC1, reduced Matrigel invasiveness by 20% and cell viability by 14%, and decreased mitogen-activated protein kinase activation in response to hepatocyte growth factor.Conclusion(s)Syndecan-1, a target of miR-10b, inhibits epithelial endometriotic cell invasiveness through down-regulation of metalloproteinase activity and interleukin-6.

My notes (saved in your browser only)

Condition tags

endometriosis

MeSH descriptors

Endometriosis Endometrium Interleukin-6 MicroRNAs Syndecan-1 Biocompatible Materials Cell Line Cell Survival Cell Survival Collagen Drug Combinations Endometriosis Endometriosis Endometriosis Endometrium Female Humans Interleukin-6 Laminin MAP Kinase Signaling System

Citation neighborhood

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

References (57)

Cited by (25)

Source provenance

europepmc
last seen: 2026-07-30T06:25:42.655704+00:00
openalex
last seen: 2026-06-10T17:14:06.276822+00:00
pubmed
last seen: 2026-05-13T22:15:58.344756+00:00
License: CC0 · commercial use OK