Comparative proteomic analysis of human adenomyosis using two-dimensional gel electrophoresis and mass spectrometry

Haiyuan Liu, Liu H, Jinghe Lang, Lang J, Xiaorong Wang, Wang X, Wu S, Shuzhen Wu
Fertility and sterility · 2007 · vol. 89(6) , pp. 1625–1631 · doi:10.1016/j.fertnstert.2007.06.081 · PMID:17884045 · W2029931927
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This study performed comparative proteomic analysis of human adenomyosis tissue using two-dimensional gel electrophoresis and mass spectrometry to identify differentially expressed proteins.

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Abstract

OBJECTIVE: To identify the differently expressed proteins in adenomyotic tissue compared with normal uterine muscle. DESIGN: Retrospective, case-controlled study. SETTING: University-based Department of Obstetrics and Gynecology and Proteomic Research Center. PATIENT(S): Ten patients who underwent transabdominal hysterectomy were included in this study. Five of them were histologically diagnosed with adenomyosis and the other five age-matched patients had cervical carcinoma in situ. INTERVENTION(S): Postoperative adenomyotic tissue and normal uterine muscle samples were collected. MAIN OUTCOME MEASURE(S): The protein extracts from paired sample tissue were separated using two-dimensional gel electrophoresis. The differently expressed protein spots were detected by gel comparison using image analysis software and identified using matrix-assisted laser desorption/ionization time of flight mass spectrometry. RESULT(S): Compared with protein profiling of normal uterine muscle, there were 12 protein spots dysregulated in adenomyotic tissue. Among them, 10 protein spots were successfully identified by mass spectrometry. CONCLUSION(S): Comparative proteomic analysis is a useful approach for the discovery of protein biomarkers for adenomyosis and understanding the pathophysiology of the disease.

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Condition tags

endometriosisadenomyosis

MeSH descriptors

Endometriosis Proteins Proteome Proteome Adult Adult Electrophoresis, Gel, Two-Dimensional Electrophoresis, Gel, Two-Dimensional Electrophoresis, Gel, Two-Dimensional Endometriosis Endometriosis Female Female Gene Expression Profiling Gene Expression Profiling Humans Humans Menstrual Cycle Menstrual Cycle Middle Aged

Citation neighborhood (2-hop)

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. Outer rings show 2-hop neighbours — papers reached through the immediate citers/citees. [ collapse to 1-hop ]

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Cited by (10)

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