Alterations of CD4+T Cell Subsets in Blood and Peritoneal Fluid in Different Stages of Endometriosis.

Fatemeh Pashizeh, Reza Mansouri, Fatemeh Davari Tanha, Fatemeh Davari-Tanha, Reyhaneh Hosseini, Zahra Asgari, Hamideh Aghaei, Farangis Najafi Arbastan, Samira Rajaei
International journal of fertility & sterility · 2020 · vol. 14(3) , pp. 201–208 · doi:10.22074/ijfs.2020.6127 · PMID:33098386 · W3094448214
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⚙ AI-generated summary by claude@2026-06, 2026-06-09 ⓘ

Advanced endometriosis patients showed increased Th17 cells in peritoneal fluid, while early endometriosis patients had more Th2 cells in peritoneal fluid compared to advanced stages, exclusively in the luteal phase.

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⚙ AI-generated deep summary by claude@2026-06, 2026-06-09 · read from full text ⓘ

This case-control study compared frequencies of CD4+ T-cell subsets (Th1, Th2, Th17, and regulatory T cells) in peripheral blood and peritoneal fluid collected from women undergoing laparoscopy across early endometriosis (stages I–II), advanced endometriosis (stages III–IV), and controls without endometriosis, using flow cytometry. No significant differences in Th1, Th2, or Th17 frequencies were observed in peripheral blood between groups, while peritoneal fluid from patients with advanced endometriosis showed increased Th17 cells versus controls. The luteal phase in early disease showed increased Th2 in peritoneal fluid compared with advanced stages, and early-stage endometriosis showed higher peritoneal fluid Th2, Th17, and Treg compared with blood; the authors also report differences between peritoneal fluid and blood in controls. This paper is centrally about endometriosis — it specifically analyzes CD4+ T-cell subset alterations in blood and peritoneal fluid across different stages of endometriosis.

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Abstract

BACKGROUND: Endometriosis is a chronic inflammatory disorder with known immune disturbances. The aim of this study was to compare the frequency of different CD4+ T cells [T helper (Th)1, Th2, Th17 and regulatory T cells (Tregs)] in peripheral blood (PB) and peritoneal fluid (PF) of patients that have early and advanced stages of endometriosis with a control group. MATERIALS AND METHODS: In this case control study, PB and PF samples were collected from women aged 24-40 years who underwent laparoscopy procedures. The frequency of CD4+ T subsets were analysed by flow cytometry and compared between three study groups; early endometriosis (stage I, II), advanced endometriosis (stage III, IV) and control (no endometriosis). T cell numbers were compared between the PB and PF in each of the aforementioned groups. RESULTS: No statistically significant difference was found between the study groups regarding the numbers of Th1, Th2 and Th17 cells in PB. The PF of patients with advanced endometriosis had increased numbers of Th17 cells compared to the control group (P=0.003), with P values of 0.059 and 0.045 in both menstrual phases. Increased numbers of Th2 cells in PF from early compared to advanced stages of endometriosis were detected exclusively in the luteal phase (P=0.035). The control group had increased numbers of Treg and Th2 cells in the PF compared to PB (both, P value=0.046). However, in the early stages of endometriosis there were more Th2, Th17 and Treg cells in the PF compared to PB (P values: 0.005, 0.047 and 0.013, respectively), while the number of Th17 cells was higher in the PF compared with PB in the advanced stages of endometriosis (P= 0.013). CONCLUSION: There were increased numbers of Th17 cells in the PF of patients with advanced stages of endometriosis, which could be related to the severity of this disease.
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International Journal of Fertility and Sterility (Oct 2020) Alterations of CD4+T Cell Subsets in Blood and Peritoneal Fluid in Different Stages of Endometriosis Abstract Background: Endometriosis is a chronic inflammatory disorder with known immune disturbances. The aim of thisstudy was to compare the frequency of different CD4+ T cells [T helper (Th)1, Th2, Th17 and regulatory T cells(Tregs)] in peripheral blood (PB) and peritoneal fluid (PF) of patients that have early and advanced stages of endometriosiswith a control group.Materials and Methods: In this case control study, PB and PF samples were collected from women aged 24-40 yearswho underwent laparoscopy procedures. The frequency of CD4+ T subsets were analysed by flow cytometry and comparedbetween three study groups; early endometriosis (stage I, II), advanced endometriosis (stage III, IV) and control(no endometriosis). T cell numbers were compared between the PB and PF in each of the aforementioned groups.Results: No statistically significant difference was found between the study groups regarding the numbers of Th1, Th2and Th17 cells in PB. The PF of patients with advanced endometriosis had increased numbers of Th17 cells comparedto the control group (P=0.003), with P values of 0.059 and 0.045 in both menstrual phases. Increased numbers of Th2cells in PF from early compared to advanced stages of endometriosis were detected exclusively in the luteal phase(P=0.035).The control group had increased numbers of Treg and Th2 cells in the PF compared to PB (both, P value=0.046).However, in the early stages of endometriosis there were more Th2, Th17 and Treg cells in the PF compared to PB (p values: 0.005, 0.047 and 0.013, respectively), while the number of Th17 cells was higher in the PF compared with PBin the advanced stages of endometriosis (P= 0.013).Conclusion: There were increased numbers of Th17 cells in the PF of patients with advanced stages of endometriosis,which could be related to the severity of this disease. Keywords

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