Interleukin-10 attenuates TNF-α–induced interleukin-6 production in endometriotic stromal cells

Yukiko Tagashira, Tagashira Y, Fuminori Taniguchi, Taniguchi F, Harada T, Tasuku Harada, Ayako Ikeda, Ikeda A, Ayako Watanabe, Watanabe A, Terakawa N, Naoki Terakawa
Fertility and sterility · 2008 · vol. 91(5) , pp. 2185–2192 · doi:10.1016/j.fertnstert.2008.04.052 · PMID:18684450 · W2032057531
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Interleukin-10 was found to reduce tumor necrosis factor-alpha-induced interleukin-6 production in endometriotic stromal cells.

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Abstract

ObjectiveTo determine whether high levels of interleukin (IL)-10 can attenuate the production of tumor necrosis factor (TNF)-alpha-induced proinflammatory cytokines in endometriotic stromal cells.DesignProspective study.SettingDepartment of Ob/Gyn, Tottori University, Japan.Patient(s)Thirty-five patients with ovarian endometrioma and ten patients with uterine myoma.Intervention(s)Endometriotic stromal cells were obtained from chocolate cyst linings of ovaries. Endometrial stromal cells obtained from patient with uterine myoma.Main outcome measure(s)Expression of IL-10 gene in endometriotic or endometrial stromal cells was determined by real-time reverse-transcriptase polymerase chain reaction (RT-PCR). We performed immunohistochemical staining to find the presence of IL-10 and IL-10 receptors 1 and 2. We examined the effects of TNF-alpha and IL-10 on the expression of IL-6 or IL-8 by real-time RT-PCR and ELISA. We examined the activation of intracellular signal transduction molecules in endometriotic stromal cells by Western blotting.Result(s)Addition of IL-10 suppressed the expressions of IL-6 induced by TNF-alpha and IL-10 induced the phosphorylation of STAT3 in endometriotic stromal cells. TNF-alpha induced the expression of phosphorylated ERK1/2, JNK1/2, and I kappaB. Adding IL-10 suppressed the phosphorylation of these signal molecules.Conclusion(s)Interleukin-10 attenuates TNF-alpha-induced IL-6 synthesis via NF-kappaB and MAPK pathways in endometriotic cells.. Interleukin-10 may play a significant role in the pathogenesis of endometriosis.

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Condition tags

endometriosis

MeSH descriptors

Endometriosis Interleukin-10 Interleukin-6 Leiomyoma Ovarian Neoplasms Stromal Cells Tumor Necrosis Factor-alpha DNA Primers Endometriosis Endometriosis Enzyme-Linked Immunosorbent Assay Female Humans Interleukin-10 Interleukin-10 Interleukin-10 Interleukin-6 Interleukin-6 Interleukin-6 Inhibitors Leiomyoma

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