Survivin, MMP-2, MT1-MMP, and TIMP-2: their impact on survival, implantation, and proliferation of endometriotic tissues

In order to study survivin, matrix metalloproteinases (MMP-2), membranous type 1 matrix metalloproteinase (MT1-MMP), and tissue inhibitor metalloproteinase-2 (TIMP-2) expression immunohistochemically in endometriotic tissues and normal endometrium, our retrospective study considered 194 patients affected by endometriosis and 71 patients with normal endometrium. Tissue microarrays were created from paraffin-embedded blocks; immunohistochemistry was used to assess protein expression. In endometriotic tissues, survivin was expressed at a higher level than in normal endometrium; its glandular expression level was higher in non-ovarian than in ovarian endometriotic tissues and lower in stromal components. Endometrial tissues from women without endometriosis and endometriotic tissues had different matrix metalloproteinase expression profiles. MMP-2 and MT1-MMP correlated with TIMP-2 in endometriotic tissues. Furthermore, in endometriotic tissues, expression of survivin, aurora B kinase, and Ki-67 showed a significant positive correlation, which indicates a role in cellular proliferation that could be closely linked to its anti-apoptotic activity in endometriosis development. Our results imply a role for matrix metalloproteinases in endometriosis invasiveness; correlation of their expression with that of TIMP-2 underscores its possible key regulatory role. Similar content being viewed by others Abbreviations - ABK: - Aurora B kinase - APC: - Adenomatous polyposis coli - ASRM: - American Society of Reproductive Medicine - ET: - Endometriotic tissue - IAP: - Inhibitor of apoptosis protein - IQR: - Interquartile range - MMP-2: - Matrix metalloproteinase-2 - MMP: - Matrix metalloproteinase - MT1-MMP: - Membranous type 1 matrix metalloproteinase - PR: - Progesterone receptor - TIMP-2: - Tissue inhibitor metalloproteinase-2 - TMA: - Tissue microarray

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Author information Authors and Affiliations Corresponding author Additional information Ambrogio P. Londero and Angelo Calcagno equally contributed to this work. Electronic supplementary material Below is the link to the electronic supplementary material. ESM 1 (download PDF ) (PDF 215 kb) Rights and permissions About this article Cite this article Londero, A.P., Calcagno, A., Grassi, T. et al. Survivin, MMP-2, MT1-MMP, and TIMP-2: their impact on survival, implantation, and proliferation of endometriotic tissues. Virchows Arch 461, 589–599 (2012). https://doi.org/10.1007/s00428-012-1301-4 Received: Revised: Accepted: Published: Issue date: DOI: https://doi.org/10.1007/s00428-012-1301-4