Tyrosine receptor kinase B (TrkB) protein expression in the human endometrium

Dana L Anger, Bingjun Zhang, Odette Boutross-Tadross, Odette Boutross‐Tadross, Warren G Foster
Endocrine · 2007 · vol. 31(2) , pp. 167–173 · doi:10.1007/s12020-007-0025-8 · PMID:17873329 · W1972192127
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TrkB protein was found in human endometrium, with higher expression in women with endometriosis compared to controls.

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This study investigated whether tyrosine receptor kinase B (TrkB) protein is expressed in eutopic human endometrium and whether its protein levels differ between women with endometriosis and healthy controls, using Western blotting (n=50) and immunohistochemistry on paraffin-embedded tissue (n=17). TrkB protein expression was significantly higher in immunoblots from women with endometriosis than from women without, and immunohistochemistry localized TrkB to the cytoplasm of epithelial cells in both groups, with higher expression suggested in those with endometriosis. The authors’ conclusion is limited by the observational design and sample sizes for each method, and the work primarily measures protein expression without directly testing functional effects. This paper is centrally about endometriosis — it measures TrkB protein expression in eutopic endometrium and finds higher TrkB levels in women with endometriosis.

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Abstract

Tyrosine kinase receptor B (TrkB) gene expression, a neurotrophic factor receptor expressed in the brain and ovary, has recently been identified in deep infiltrating endometriosis by gene array. TrkB is thought to be important in resistance to anchorage independent apoptosis (ANOIKIS) and thus could be important in the pathogenesis of endometriosis. However, TrkB protein expression in the eutopic endometrium of women with and without endometriosis is unknown. Therefore, we examined TrKB protein expression in the endometrium by Western blot (n = 50) and immunohistochemistry (n = 17). Immunoblots of endometrial biopsies were prepared from women with endometriosis (n = 21) vs. healthy controls (n = 29) undergoing benign gynecological surgery at McMaster University Medical Centre. TrkB protein expression was significantly higher in immunoblots from women with endometriosis compared to women without endometriosis. In samples of archived paraffin-embedded endometrial tissue TrkB was localized to the cytoplasm of epithelial cells of the eutopic endometrium from women with endometriosis (n = 7) and without endometriosis (n = 10). We conclude that TrkB protein is expressed in human endometrium. Our results also suggest that TrkB expression may be greater in women with endometriosis compared to women without endometriosis. Similar content being viewed by others

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Author information Authors and Affiliations Corresponding author Rights and permissions About this article Cite this article Anger, D.L., Zhang, B., Boutross-Tadross, O. et al. Tyrosine receptor kinase B (TrkB) protein expression in the human endometrium. Endocr 31, 167–173 (2007). https://doi.org/10.1007/s12020-007-0025-8 Received: Revised: Accepted: Published: Issue date: DOI: https://doi.org/10.1007/s12020-007-0025-8

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endometriosis

MeSH descriptors

Endometrium Receptor, trkB Adult Endometriosis Endometriosis Endometriosis Endometriosis Endometrium Endometrium Female Gene Expression Humans Middle Aged Receptor, trkB Receptor, trkB

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