{"paper_id":"928903f4-7b4d-45fd-beb4-6c251ff13f33","body_text":"Abstract\nTyrosine kinase receptor B (TrkB) gene expression, a neurotrophic factor receptor expressed in the brain and ovary, has recently been identified in deep infiltrating endometriosis by gene array. TrkB is thought to be important in resistance to anchorage independent apoptosis (ANOIKIS) and thus could be important in the pathogenesis of endometriosis. However, TrkB protein expression in the eutopic endometrium of women with and without endometriosis is unknown. Therefore, we examined TrKB protein expression in the endometrium by Western blot (n = 50) and immunohistochemistry (n = 17). Immunoblots of endometrial biopsies were prepared from women with endometriosis (n = 21) vs. healthy controls (n = 29) undergoing benign gynecological surgery at McMaster University Medical Centre. TrkB protein expression was significantly higher in immunoblots from women with endometriosis compared to women without endometriosis. In samples of archived paraffin-embedded endometrial tissue TrkB was localized to the cytoplasm of epithelial cells of the eutopic endometrium from women with endometriosis (n = 7) and without endometriosis (n = 10). We conclude that TrkB protein is expressed in human endometrium. Our results also suggest that TrkB expression may be greater in women with endometriosis compared to women without endometriosis.\nSimilar content being viewed by others\nReferences\nR.L. Barbieri, Am. J. Obstet. Gynecol. 162(2), 565–567 (1990)\nF.M. Howard, Obstet. Gynecol. Surv. 48(6), 357–387 (1993)\nJ. Sampson, Am. J. Obstet. Gynecol. 14, 422–469 (1927)\nD.P. Braun, W.P. Dmowski, Curr. Opin. Obstet. Gynecol. 10(5), 365–369 (1998)\nM. Nisolle, F. Casanas-Roux, J. Donnez, Fertil. Steril. 74(2), 306–312 (2000)\nA. Beliard, A. Noel, F. Goffin, F. Frankenne, J.M. Foidart, Fertil. Steril. 78(5), 973–978 (2002)\nJ. Donnez, P. Smoes, S. Gillerot, F. Casanas-Roux, M. Nisolle, Hum. Reprod. 13(6), 1686–1690 (1998)\nA. Akoum, A. Lemay, C. Brunet, J. Hebert, Fertil. Steril. 63, 322–328 (1995)\nA. Akoum, C.N. Metz, M. Al-Akoum, R. Kats, Fertil. Steril. 85(5), 1379–1385 (2006)\nA. Kharfi, A. Boucher, A. Akoum, Biol. Reprod. 66, 401–406 (2002)\nA. Beliard, A. Noel, J.M. Foidart, Fertil. Steril. 82(1), 80–85 (2004)\nD.P. Braun, J. Ding, J. Shen, N. Rana, B.B. Fernandez, W.P. Dmowski, Fertil. Steril. 78(4), 830–835 (2002)\nW.P. Dmowski, J. Ding, J. Shen, N. Rana, B.B. Fernandez, D.P. Braun, Hum. Reprod. 16(9), 1802–1808 (2001)\nW.P. Dmowski, H. Gebel, D.P. Braun, Hum. Reprod. Update. 4(5), 696–701 (1998)\nH.M. Gebel, D.P. Braun, A. Tambur, D. Frame, N. Rana, W.P. Dmowski, Fertil. Steril. 69(6), 1042–1047 (1998)\nN.M. Zamah, M.G. Dodson, L.C. Stephens, V.C. Buttram Jr., P.K. Besch, R.H. Kaufman, Am. J. Obstet. Gynecol. 149(6), 591–597 (1984)\nB. Lessey, A. Castlebaum, S. Sawin, C. Buck, R. Schinnar, W. Bilker, B. Strom, J. Clin. Endocrinol. Metab. 79, 643–649 (1994)\nV. Rai, J. Hopkisson, S. Kennedy, A. Bergqvist, D.H. Barlow, H.J. Mardon, J. Pathol. 180(2), 181–187 (1996)\nMdel M. Vernet-Tomas, C.T. Perez-Ares, N. Verdu, M.T. Fernandez-Figueras, J.L. Molinero, R. Carreras, J. Soc. Gynecol. Investig. 13(4), 292–296 (2006)\nP.J.Q. van der Linden, E.P.M. van der Linden, A.F.P.M. de Goeij, F.C.S. Ramaekers, G.A.J. Dunselman, J.L.H. Evers, Fertil. Steril. 61, 85–90 (1994)\nS.E. Rier, P.N. Zarmkoupis, X. Hu, J.L. Becker, J. Clinical Endocrinology & Metabolism 80, 1431–1437 (1995)\nL.S. Noble, E.R. Simpson, A. Johns, S.E. Bulun, J. Clin. Endocrinol. Metab 81(1), 174–179 (1996)\nE. Attar, S.E. Bulun, Fertil. Steril. 85(5), 1307–1318 (2006)\nW.C. Keetel, R.J. Stein, Am. J. Obstet. Gynecol. 61(440), 442 (1951)\nD. Bartosik, S.L. Jacobs, L.J. Kelly, Fertil. Steril. 46(5), 796–800 (1986)\nM.C. Johnson, M. Torres, A. Alves, K. Bacallao, A. Fuentes, M. Vega, M.A. Boric, Reprod. Biol. Endocrinol. 3, 45 (2005)\nG.F. Meresman, S. Vighi, R.A. Buquet, O. Contreras-Ortiz, M. Tesone, L.S. Rumi, Fertil. Steril. 74(4), 760–766 (2000)\nR.K. Jones, R.F. Searle, J.N. Bulmer, Hum. Reprod. 13(12), 3496–3502 (1998)\nS. Douma, T. Van Laar, J. Zevenhoven, R. Meuwissen, E. Van Garderen, D.S. Peeper, Nature 430(7003), 1034–1039 (2004)\nS. Matsuzaki, M. Canis, C. Vaurs-Barriere, J.L. Pouly, O. Boespflug-Tanguy, F. Penault-Llorca, P. Dechelotte, B. Dastugue, K. Okamura, G. Mage, Mol. Hum. Reprod. 10(10), 719–728 (2004)\nR. Klein, L.F. Parada, F. Coulier, M. Barbacid, EMBO J. 8(12), 3701–3709 (1989)\nS. Matsuzaki, M. Canis, J.L. Pouly, R. Botchorishvili, P.J. Dechelotte, G. Mage, Fertil. Steril. (2006)\nR.A. Anderson, L.L. Robinson, J. Brooks, N. Spears, J. Clin. Endocrinol. Metab 87(2), 890–897 (2002)\nE.Y Adashi, 62(20–27), (1994)\nD.B. Seifer, B. Feng, R.M. Shelden, S. Chen, C.F. Dreyfus, J. Clin. Endocrinol. Metab 87(2), 655–659 (2002)\nE. Shibayama, H. Koizumi, Am. J. Pathol. 148(6), 1807–1818 (1996)\nJ.M. Hastings, K.S. Jackson, P.A. Mavrogianis, A.T. Fazleabas, Biol. Reprod. 75(2), 176–182 (2006)\nR. Klein, D. Conway, L.F. Parada, M. Barbacid, Cell 61(4), 647–656 (1990)\nD.S. Middlemas, R.A. Lindberg, T. Hunter, Mol. Cell Biol. 11(1), 143–153 (1991)\nG.T. Baxter, M.J. Radeke, R.C. Kuo, V. Makrides, B. Hinkle, R. Hoang, A. Medina-Selby, D. Coit, P. Valenzuela, S.C. Feinstein, J. Neurosci. 17(8), 2683–2690 (1997)\nT. Arimoto, T. Katagiri, K. Oda, T. Tsunoda, T. Yasugi, Y. Osuga, H. Yoshikawa, O. Nishii, T. Yano, Y. Taketani, Y. Nakamura, Int. J. Oncol. 22(3), 551–560 (2003)\nM.M. Dugich-Djordjevic, F. Ohsawa, T. Okazaki, N. Mori, J.R. Day, K.D. Beck, F. Hefti, Neuroscience 66(4), 861–877 (1995)\nN. Lindefors, E. Brodin, M. Metsis, Neuroscience 65(3), 661–670 (1995)\nM. Dragunow, E. Beilharz, B. Mason, P. Lawlor, W. Abraham, P. Gluckman, Neurosci. Lett. 160(2), 232–236 (1993)\nT.J. Kingsbury, P.D. Murray, L.L. Bambrick, B.K. Krueger, J. Biol. Chem. 278(42), 40744–40748 (2003)\nA. Meyer-Franke, G.A. Wilkinson, A. Kruttgen, M. Hu, E. Munro, M.G. Hanson Jr., L.F. Reichardt, B.A. Barres, Neuron 21(4), 681–693 (1998)\nJ. Du, L. Feng, F. Yang, B. Lu, J. Cell Biol. 150(6), 1423–1434 (2000)\nA. Haapasalo, I. Sipola, K. Larsson, K.E. Akerman, P. Stoilov, S. Stamm, G. Wong, E. Castren, J. Biol. Chem. 277(45), 43160–43167 (2002)\nX. Tao, S. Finkbeiner, D.B. Arnold, A.J. Shaywitz, M.E. Greenberg, Neuron 20(4), 709–726 (1998)\nP.B. Shieh, S.C. Hu, K. Bobb, T. Timmusk, A. Ghosh, Neuron 20(4), 727–740 (1998)\nM.J. Cambiasso, J.A. Colombo, H.F. Carrer, Eur. J. Neurosci. 12(7), 2291–2298 (2000)\nM.K. Jezierski, F. Sohrabji, Neurobiol. Aging 22(2), 309–319 (2001)\nD.T. Solum, R.J. Handa, J. Neurosci. 22(7), 2650–2659 (2002)\nThe American Fertility Society, Fertil.Steril. 43(3), 351–352 (1985)\nM.M. Bradford, Anal. Biochem. 72, 248–254 (1976)\nAcknowledgments\nThe authors would like to sincerely thank the members of McMaster’s Department of Pathology for their help in obtaining endometrial biopsy samples and retrieving archived embedded samples. The technical assistance of Ms Stephanie Bulk for running the immunoblots in this study is gratefully appreciated. Financial support for this project was provided by the Natural Sciences and Engineering Research Council of Canada (261905-03 to WGF). Salary support for Dr. Foster from the CIHR and the Ontario Women’s Health Council is gratefully acknowledged.\nAuthor information\nAuthors and Affiliations\nCorresponding author\nRights and permissions\nAbout this article\nCite this article\nAnger, D.L., Zhang, B., Boutross-Tadross, O. et al. Tyrosine receptor kinase B (TrkB) protein expression in the human endometrium. Endocr 31, 167–173 (2007). https://doi.org/10.1007/s12020-007-0025-8\nReceived:\nRevised:\nAccepted:\nPublished:\nIssue date:\nDOI: https://doi.org/10.1007/s12020-007-0025-8","source_license":"CC0","license_restricted":false}