Ovarian Endometriosis Signatures Established through Discovery and Directed Mass Spectrometry Analysis

Anni P. Vehmas, Vehmas AP, Dorota Muth-Pawlak, Dorota Muth‐Pawlak, Kaisa Huhtinen, Saloniemi-Heinonen T, Taija Saloniemi-Heinonen, Kimmo Jaakkola, Jaakkola K, Teemu D. Laajala, Heidi Kaprio, Kaprio H, Suvitie PA, Pia A Suvitie, Pia Suvitie, Tero Aittokallio, Harri Siitari, Siitari H, Antti Perheentupa, Perheentupa A, Matti Poutanen, Garry L. Corthals
Journal of proteome research · 2014 · vol. 13(11) , pp. 4983–4994 · doi:10.1021/pr500384n · PMID:25099244 · W1975331975
article OA: closed CC0 ⤵ 32 in-corpus citations
View on OpenAlex View on PubMed View at publisher
⚙ AI-generated summary by gemini-2.5-flash-lite, 2026-06-07 ⓘ

This study identified 214 differentially expressed proteins between ovarian endometrioma and endometrium using mass spectrometry, revealing potential biomarkers and therapeutic targets, including Calponin-1 and EMILIN-1.

One-sentence paraphrase of the abstract; not a substitute for reading it. No clinical advice. How this works

Abstract

New molecular information on potential therapeutic targets or tools for noninvasive diagnosis for endometriosis are important for patient care and treatment. However, surprisingly few efforts have described endometriosis at the protein level. In this work we enumerate the proteins in patient endometrium and ovarian endometrioma by extensive and comprehensive analysis of minute amounts of cryosectioned tissues in a three-tiered mass spectrometric approach. Quantitative comparison of the tissues revealed 214 differentially expressed proteins in ovarian endometrioma and endometrium. These proteins are reported here as a resource of SRM (selected reaction monitoring) assays that are unique, standardized, and openly available. Pathway analysis of the proteome measurements revealed a potential role for Transforming growth factor β-1 in ovarian endometriosis development. Subsequent mRNA microarray analysis further revealed clear ovarian endometrioma specificity for a subset of these proteins, which was also supported by further in silico studies. In this process two important proteins emerged, Calponin-1 and EMILIN-1, that were additionally confirmed in ovarian endometrioma tissues by immunohistochemistry and Western blotting. This study provides the most comprehensive molecular description of ovarian endometriosis to date and researchers with new molecular methods and tools for high throughput patient screening using the SRM assays.

My notes (saved in your browser only)

Condition tags

endometriosisendometrioma

MeSH descriptors

Endometriosis Mass Spectrometry Proteins Proteins Proteomics Blotting, Western Calcium-Binding Proteins Calcium-Binding Proteins Calcium-Binding Proteins Calponins Computer Simulation Endometriosis Endometriosis Female Humans Mass Spectrometry Membrane Glycoproteins Membrane Glycoproteins Membrane Glycoproteins Microfilament Proteins

Citation neighborhood

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

References (57)

Cited by (31)

Source provenance

europepmc
last seen: 2026-09-17T06:16:55.786923+00:00
openalex
last seen: 2026-06-10T17:14:06.276822+00:00
pubmed
last seen: 2026-05-13T22:18:22.440000+00:00
unpaywall
last seen: 2026-09-17T06:32:46.214484+00:00
License: CC0 · commercial use OK