Comprehensive proteomic analysis of human endometrial fluid aspirate

Juan Casado-Vela, Juan Casado‐Vela, Eva Rodriguez-Suarez, Rodriguez-Suarez E, Eva Rodríguez‐Suárez, Ibon Iloro, Ibón Iloro, Ametzazurra A, Amagoia Ametzazurra, Alkorta N, Nere Alkorta, Juan A. García-Velasco, Juan Antonio García-Velasco, Roberto Matorras, Matorras R, Prieto B, Begoña Prieto, Sandra González, González S, Daniel Nagore, Nagore D, Simón L, Laureano Simón, Felix Elortza, Félix Elortza
Journal of proteome research · 2009 · vol. 8(10) , pp. 4622–4632 · doi:10.1021/pr9004426 · PMID:19670903 · W2027223134
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This study identified 803 proteins in human endometrial fluid during the secretory phase using three complementary proteomic strategies, establishing a reference proteome for understanding endometrial function and disease.

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Abstract

The endometrial fluid is a noninvasive sample which contains numerous secreted proteins representative of endometrial function and reflects the state of the endometrium. In this study, we describe, for the first time, a comprehensive catalogue of proteins of the endometrial fluid during the secretory phase of the menstrual cycle. To achieve this objective, three different but complementary strategies were used: First, in-solution digestion followed by reverse phase high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS); second, protein separation by denaturing one-dimensional electrophoresis (SDS-PAGE) followed by HPLC-MS/MS analysis. Finally, two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) followed by MALDI-TOF/TOF analysis. The combination of the three strategies led to the successful identification of 803 different proteins in the International Protein Index (IPI) human database (v3.48). An extensive description of the endometrial fluid proteome will help provide the basis for a better understanding of a number of diseases and processes, including endometriosis, endometrial cancer and embryo implantation. We believe that the thorough catalogue of proteins presented here can serve as a valuable reference for the study of embryo implantation and for future biomarker discovery involved in pathologic alterations of endometrial function.

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Condition tags

endometriosis

MeSH descriptors

Body Fluids Endometrium Proteome Proteomics Adolescent Adolescent Adult Adult Amino Acid Sequence Amino Acid Sequence Biopsy, Needle Biopsy, Needle Body Fluids Body Fluids Chromatography, High Pressure Liquid Chromatography, High Pressure Liquid Cluster Analysis Cluster Analysis Electrophoresis, Gel, Two-Dimensional Electrophoresis, Gel, Two-Dimensional

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Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. Outer rings show 2-hop neighbours — papers reached through the immediate citers/citees. [ collapse to 1-hop ]

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