Comprehensive Proteomic Analysis of Human Endometrial Fluid Aspirate

Juan Casado-Vela, Juan Casado‐Vela, Eva Rodriguez-Suarez, Rodriguez-Suarez E, Eva Rodríguez‐Suárez, Ibon Iloro, Ibón Iloro, Ametzazurra A, Amagoia Ametzazurra, Alkorta N, Nere Alkorta, Juan A. García-Velasco, Juan Antonio García-Velasco, Roberto Matorras, Matorras R, Prieto B, Begoña Prieto, Sandra González, González S, Daniel Nagore, Nagore D, Simón L, Laureano Simón, Felix Elortza, Félix Elortza
Journal of proteome research · 2009 · vol. 8(10) , pp. 4622–4632 · doi:10.1021/pr9004426 · PMID:19670903 · W2027223134
article OA: closed CC0 ⤵ 15 in-corpus citations
📄 Open PDF View on OpenAlex View on PubMed View at publisher
⚙ AI-generated summary by gemini-2.5-flash-lite, 2026-06-08 ⓘ

This study identified 803 proteins in human endometrial fluid during the secretory phase using three complementary proteomic strategies, establishing a reference proteome for understanding endometrial function and disease.

One-sentence paraphrase of the abstract; not a substitute for reading it. No clinical advice. How this works

Abstract

The endometrial fluid is a noninvasive sample which contains numerous secreted proteins representative of endometrial function and reflects the state of the endometrium. In this study, we describe, for the first time, a comprehensive catalogue of proteins of the endometrial fluid during the secretory phase of the menstrual cycle. To achieve this objective, three different but complementary strategies were used: First, in-solution digestion followed by reverse phase high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS); second, protein separation by denaturing one-dimensional electrophoresis (SDS-PAGE) followed by HPLC-MS/MS analysis. Finally, two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) followed by MALDI-TOF/TOF analysis. The combination of the three strategies led to the successful identification of 803 different proteins in the International Protein Index (IPI) human database (v3.48). An extensive description of the endometrial fluid proteome will help provide the basis for a better understanding of a number of diseases and processes, including endometriosis, endometrial cancer and embryo implantation. We believe that the thorough catalogue of proteins presented here can serve as a valuable reference for the study of embryo implantation and for future biomarker discovery involved in pathologic alterations of endometrial function.

My notes (saved in your browser only)

Condition tags

endometriosis

MeSH descriptors

Body Fluids Endometrium Proteome Proteomics Adolescent Adult Amino Acid Sequence Biopsy, Needle Body Fluids Chromatography, High Pressure Liquid Cluster Analysis Electrophoresis, Gel, Two-Dimensional Embryo Implantation Endometrial Neoplasms Endometriosis Endometrium Female Humans Isoelectric Focusing Luteal Phase

Citation neighborhood

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

References (67)

Cited by (15)

Source provenance

europepmc
last seen: 2026-09-04T06:17:57.233406+00:00
openalex
last seen: 2026-06-04T00:00:01.174412+00:00
pubmed
last seen: 2026-05-13T22:13:59.677786+00:00
License: CC0 · commercial use OK