Establishment of an immortalized cell line derived from human adenomyosis ectopic lesions

Wang Z, Zilu Wang, Cui F, Fengxin Cui, Chen Y, Yinuo Chen, Hongyun Liu, Liu H, Yiran Zhang, Yaxin Shi, Shi Y, Yinuo Zhang, Zhang Y, Yanfei Wang, Wang Y, Liang N, Na Liang, Xu L, Li Xu, Ying Liu, Liu Y, Zhiyong Liu, Liu Z, Wei Shi, Shi W
Tissue & cell · 2023 · vol. 86 , pp. 102284 · doi:10.1016/j.tice.2023.102284 · PMID:38134573 · W4389235885
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Researchers established an immortalized human adenomyosis ectopic cell line (ihAMEC) from primary cells, which maintained epithelial-mesenchymal transition marker expression and similar responses to hormone and LPS treatments while exhibiting active proliferation without tumor formation.

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Abstract

Because adenomyosis (AM) ectopic primary cells are hard to come by, have a short lifespan, and the characteristics that alter over time, their utility in AM research is constrained. This study aimed to establish a line of immortalized human adenomyosis ectopic cell (ihAMEC) to change this situation. Primary cells were obtained from AM ectopic lesion tissue and then infected with Simian Vacuolating Virus 40 Tag (SV40 T) lentivirus and screened to establish immortalized cells. We verified the main features and found that the ihAMEC could be cultured for more than 50 generations and the proliferation ability of ihAMEC was more active than that of primary cells. The cytoskeleton and cell types of ihAMEC were similar to primary cells and maintained a normal karyotype. The expression of epithelial-mesenchymal transition (EMT) markers, estrogen-metabolizing proteins, and estrogen/progesterone receptors in ihAMEC was similar to the expression seen in primary cells. In addition, the response of ihAMEC under estrogen treatment and Lipopolysaccharide intervention is similar to primary cells. The clonogenic ability of ihAMEC was lower than tumor cells and did not form tumors in tumorigenicity assays. Thus, ihAMEC can be used as in vitro cellular model for pathogenesis and drug development studies regarding AM.

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Condition tags

adenomyosis

MeSH descriptors

Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis

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Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. Outer rings show 2-hop neighbours — papers reached through the immediate citers/citees. [ collapse to 1-hop ]

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