An epigenetic disorder may cause aberrant expression of aromatase gene in endometriotic stromal cells

Izawa M, Masao Izawa, Tasuku Harada, Harada T, Taniguchi F, Fuminori Taniguchi, Yoko Ohama, Ohama Y, Takenaka Y, Yasuko Takenaka, Terakawa N, Naoki Terakawa
Fertility and sterility · 2007 · vol. 89(5) , pp. 1390–1396 · doi:10.1016/j.fertnstert.2007.03.078 · PMID:17662285 · W2038692288
article OA: bronze CC0 ⤵ 60 in-corpus citations
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This paper investigated potential epigenetic dysregulation leading to abnormal aromatase gene expression within endometriotic stromal cells.

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Abstract

ObjectiveTo examine the molecular basis of aromatase expression in stromal cell culture from endometriotic chocolate cysts.DesignProspective study.SettingDepartment of Obstetrics and Gynecology and Department of Biosignaling, Tottori University, Yonago Japan.Patient(s)Thirty women, selected randomly, who underwent laparoscopy (n = 18) or laparotomy (n = 12).Intervention(s)Endometrial and endometriotic stromal cells were obtained from the uterus and chocolate cyst lining of the ovary.Main outcome measure(s)Estradiol concentrations in the culture media were measured by means of enzyme immunoassay. Aromatase expression was examined by quantitative real-time polymerase chain reaction. Promoter usage was examined using unique exon I (PII, I.1, I.3, I.4, I.5, and I.6) and exon II primers. To determine the effect of 5-aza-deoxycytidine on endometrial stromal cells, the cells were treated with the agent for 96 hours.Result(s)Endometriotic cells secreted a marginal level of estradiol into the culture media, but adding testosterone to the culture produced a pronounced level of estradiol. In endometrial cells, estradiol production was far less efficient than in endometriotic cells even after adding testosterone. Real-time polymerase chain reaction analyses demonstrated the up-regulation of aromatase messenger RNA (mRNA) expression in endometriotic cells. Three proximal promoters, PII, 1.3, and 1.6, drove mRNA expression. In endometrial cells where a marginal level of aromatase mRNA expression was observed, the same promoters as those in the endometriotic cells were used. To determine the role of epigenetic modification of aromatase gene expression in endometriotic cells, endometrial cells were treated with 5-aza-deoxycytidine, which markedly enhanced aromatase mRNA expression, depending on the same proximal promoters as those in endometriotic cells.Conclusion(s)An epigenetic disorder may play a role in the pathophysiology of endometriosis.

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Condition tags

endometriosis

MeSH descriptors

Aromatase Endometriosis Epigenesis, Genetic Gene Expression Regulation, Enzymologic Ovarian Diseases Stromal Cells Uterine Diseases Aromatase Aromatase Azacitidine Azacitidine Azacitidine Cells, Cultured CpG Islands CpG Islands Decitabine DNA Methylation Endometriosis Endometriosis Endometriosis

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References (29)

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