High-throughput mRNA sequencing of stromal cells from endometriomas and endometrium

Rekker K, Kadri Rekker, Merli Saare, Elo Eriste, Eriste E, Tõnis Tasa, Viktorija Kukuškina, Kukuškina V, Roost AM, Anne Mari Roost, Kristi Bøgh Anderson, Kristi Anderson, Samuel K, Külli Samuel, Helle Karro, Karro H, Salumets A, Andres Salumets, Maire Peters
Reproduction (Cambridge, England) · 2017 · vol. 154(1) , pp. 93–100 · doi:10.1530/rep-17-0092 · PMID:28495852 · W2613397701
Lab / animal OA: bronze CC0 ⤵ 25 in-corpus citations
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High-throughput mRNA sequencing of endometrioma stromal cells identified over 1300 dysregulated genes and highlighted major imbalances in complement regulation compared to eutopic endometrial stromal cells.

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Abstract

The aetiology of endometriosis is still unclear and to find mechanisms behind the disease development, it is important to study each cell type from endometrium and ectopic lesions independently. The objective of this study was to uncover complete mRNA profiles in uncultured stromal cells from paired samples of endometriomas and eutopic endometrium. High-throughput mRNA sequencing revealed over 1300 dysregulated genes in stromal cells from ectopic lesions, including several novel genes in the context of endometriosis. Functional annotation analysis of differentially expressed genes highlighted pathways related to cell adhesion, extracellular matrix-receptor interaction and complement and coagulation cascade. Most importantly, we found a simultaneous upregulation of complement system components and inhibitors, indicating major imbalances in complement regulation in ectopic stromal cells. We also performed in vitro experiments to evaluate the effect of endometriosis patients' peritoneal fluid (PF) on complement system gene expression levels, but no significant impact of PF on C3, CD55 and CFH levels was observed. In conclusion, the use of isolated stromal cells enables to determine gene expression levels without the background interference of other cell types. In the future, a new standard design studying all cell types from endometriotic lesions separately should be applied to reveal novel mechanisms behind endometriosis pathogenesis.

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Condition tags

endometriosis

MeSH descriptors

Biomarkers Endometriosis Endometrium High-Throughput Nucleotide Sequencing RNA, Messenger Stromal Cells Adult Adult Biomarkers Biomarkers Case-Control Studies Case-Control Studies Endometriosis Endometriosis Endometriosis Endometrium Endometrium Female Female Gene Expression Profiling

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