The protein kinase A pathway-regulated transcriptome of endometrial stromal fibroblasts reveals compromised differentiation and persistent proliferative potential in endometriosis

Lusine Aghajanova, Horcajadas JA, Jose A Horcajadas, J.A. Horcajadas, Weeks JL, James L. Weeks, Francisco J. Esteban, Camran Nezhat, Camran N Nezhat, Nezhat CN, Conti M, Marco Conti, Giudice LC, Linda C. Giudice
Endocrinology · 2010 · vol. 151(3) , pp. 1341–1355 · doi:10.1210/en.2009-0923 · PMID:20068008 · PMC2840687 · W2031316279
Lab / animal OA: bronze CC0 ⤵ 51 in-corpus citations
📄 Open PDF View on OpenAlex View on PubMed View at publisher
⚙ AI-generated summary by gemini-2.5-flash-lite, 2026-06-07 ⓘ

Endometrial stromal fibroblasts from women with endometriosis exhibit a blunted response to protein kinase A pathway activation, showing compromised differentiation and a persistent proliferative phenotype compared to those without endometriosis.

One-sentence paraphrase of the abstract; not a substitute for reading it. No clinical advice. How this works

Abstract

Intrinsic abnormalities in transplanted eutopic endometrium are believed to contribute to the pathogenesis of pelvic endometriosis. Herein we investigated transcriptomic differences in human endometrial stromal fibroblasts (hESFs) from women with (hESF(endo)) vs. without (hESF(nonendo)) endometriosis, in response to activation of the protein kinase A (PKA) pathway with 8-bromoadenosine-cAMP (8-Br-cAMP). hESF(nonendo) (n = 4) and hESF(endo) (n = 4) were isolated from eutopic endometrium and treated +/- 0.5 mm 8-Br-cAMP for 96 h. Purified total RNA was subjected to microarray analysis using the whole-genome Gene 1.0 ST Affymetrix platform. A total of 691 genes were regulated in cAMP-treated hESF(nonendo) vs. 158 genes in hESF(endo), suggesting a blunted response to cAMP/PKA pathway activation in women with disease. Real-time PCR and ELISA validated the decreased expression of decidualization markers in hESF(endo) compared with hESF(nonendo). In the absence of disease, 8-Br-cAMP down-regulated progression through the cell cycle via a decrease in cyclin D1, cyclin-dependent kinase 6, and cell division cycle 2 and an increase in cyclin-dependent kinase inhibitor 1A. However, cell cycle components in hESF(endo) were not responsive to 8-Br-cAMP, resulting in persistence of a proliferative phenotype. hESF(endo) treated with 8-Br-cAMP exhibited altered expression of immune response, extracellular matrix, cytoskeleton, and apoptosis genes. Changes in phosphodiesterase expression and activity were not different among experimental groups. These data support that eutopic hESF(endo) with increased proliferative potential can seed the pelvic cavity via retrograde menstruation and promote establishment, survival, and proliferation of endometriosis lesions, independent of hydrolysis of cAMP and likely due to an inherent abnormality in the PKA pathway.

My notes (saved in your browser only)

Condition tags

endometriosis

MeSH descriptors

Cell Cycle Cell Cycle Cell Differentiation Cell Differentiation Cyclic AMP-Dependent Protein Kinases Endometriosis Gene Expression Profiling Gene Expression Profiling Adult Adult Case-Control Studies Case-Control Studies Cell Proliferation Cell Proliferation Cyclic AMP-Dependent Protein Kinases Cyclic AMP-Dependent Protein Kinases Cyclins Cyclins Cyclins Cytoskeletal Proteins

Citation neighborhood (2-hop)

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. Outer rings show 2-hop neighbours — papers reached through the immediate citers/citees. [ collapse to 1-hop ]

References (71)

Cited by (50)

Source provenance

europepmc
last seen: 2026-10-17T06:09:56.316927+00:00
openalex
last seen: 2026-06-10T17:14:06.276822+00:00
pubmed
last seen: 2026-10-08T21:07:11.085580+00:00
License: CC0 · commercial use OK