COX-2 gene promoter DNA methylation status in eutopic and ectopic endometrium of Egyptian women with endometriosis

Zidan HE, Haidy E. Zidan, Noha A. Rezk, Rezk NA, Amr Abd Almohsen Alnemr, Alnemr AA, Amany M. Abd el Ghany, Abd El Ghany AM
Journal of Reproductive Immunology · 2015 · vol. 112 , pp. 63–67 · doi:10.1016/j.jri.2015.06.093 · PMID:26276091 · W992937218
Non-randomised trial OA: closed CC0 ⤵ 26 in-corpus citations
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This study investigated the DNA methylation status of the COX-2 gene promoter in eutopic and ectopic endometrial tissues from Egyptian women diagnosed with endometriosis.

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Abstract

The pathophysiology of COX-2 expression in endometriosis is a matter of debate. The aim was to investigate the role of DNA methylation of the NF-IL6 site within the promoter of COX-2 gene in the pathogenesis of endometriosis. The endometrial tissues (ectopic and eutopic) were collected from 60 women with endometriosis and 30 women without endometriosis (control group). The methylation status of COX-2 was examined by methylation-specific PCR. Quantitative real-time PCR (RT-PCR) was performed to measure COX-2 mRNA levels in endometrial tissues. We found significantly higher levels of COX-2 in ectopic endometriotic tissue compared with eutopic tissue. Also, we found that the frequencies of methylation status of the NF-IL6 site within the COX-2 promoter in the eutopic and ectopic endometrial tissues of endometriosis groups were significantly decreased in comparison to controls (P=0.002, P=0.000 respectively). Our study demonstrated that DNA hypomethylation of the NF-IL6 site within the promoter of COX-2 gene could be a key mechanism for its elevated expression in the eutopic and ectopic tissues of endometriosis.

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Condition tags

endometriosis

MeSH descriptors

Cyclooxygenase 2 DNA Methylation DNA Methylation Endometriosis Endometrium Promoter Regions, Genetic Promoter Regions, Genetic Adult Adult CCAAT-Enhancer-Binding Protein-beta CCAAT-Enhancer-Binding Protein-beta CCAAT-Enhancer-Binding Protein-beta Cyclooxygenase 2 Cyclooxygenase 2 Cyclooxygenase 2 Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis

Citation neighborhood (2-hop)

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References (40)

Cited by (26)

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