Endometriotic Cells Exhibit Metaplastic Change and Oxidative DNA Damage as Well as Decreased Function, Compared to Normal Endometrium

Summary A widely accepted theory of the etiology of endometriosis is that it originates from the implantation and invasion of cells from retrograde menstruation to various sites in the body particularly the pelvic peritoneal cavity. Little is known of the function of these cells in ectopic sites. Normal endometrium was compared with endometriotic tissue using an antibody to Placental Cadherin (P Cadherin), a recently studied cadherin that is implicated in metaplasia and early neoplasia and also 8-hydroxyguanine, an indicator of oxidative DNA damage. Comparisons of endometrial tissue function were made using expression of transforming growth factor β-1 (TGFβ-1) and insulin-like growth factor-I (IGF-I). There was no labelling for anti-P Cadherin or anti-8-hydroxydeoxyguanosine in normal endometrium but marked labelling for both on the apical surface of the endometriotic epithelium. Studies of markers of normal endometrial function were all de-expressed in endometriosis. This study indicates that endometriosis cells are abnormal and exhibit oxidative DNA damage, metaplasia and markedly reduced function compared to normal endometrium. Similar content being viewed by others References C Alvarez-Rodriguez LA Baiza-Guzman (1996) ArticleTitleRegulation of uterine cellular proliferation with estrogens and growth factors Ginecol Obstet Mex 64 406–410 Occurrence Handle8964542 M Bush J Mele G Couchman D Walmer (1998) ArticleTitleEvidence of juxtacrine signaling for transforming growth factor alpha in human endometrium Biol Reprod 59 1522–1529 Occurrence Handle9828201 I Caniggia CV Taylor JW Ritchie SJ Lye M Letarte (1997) ArticleTitleEndoglin regulates trophoblast differentiation along the invasive pathway in human placental villous explants Endocrinology 138 4977–4988 Occurrence Handle10.1210/en.138.11.4977 Occurrence Handle9348229 SY Chang YS Ho (1997) ArticleTitleImmunohistochemical analysis of insulin-like growth factor I, insulin-like growth factor I receptor and insulin-like growth factor II in endometriotic tissue and endometrium Acta Obstet Gynecol Scand 76 112–117 Occurrence Handle9049281 GT Chen CT Tai LS Yeh TC Yang HD Tsai (2002) ArticleTitleIdentification of the cadherin subtypes present in the human peritoneum and endometriotic lesions: potential role for P cadherin in the delopment of endometriosis Mole Reprod Dev 62 289–294 Occurrence Handle10.1002/mrd.10121 CC Chiou PY Chang EC Chan TL Wu KC Tsao JT Wu (2003) ArticleTitleUrinary 8-hydroxydeoxyguanosine and its analogs as DNA markers of oxidative stress: development of an ELISA and measurement in both bladder and prostate cancers Clin Chim Acta 334 87–94 Occurrence Handle10.1016/S0009-8981(03)00191-8 Occurrence Handle12867278 SK Das H Lim J Wang BC Paria M BazDresch SK Dey (1997) ArticleTitleInappropriate expression of human transforming growth factor (TGF)-alpha in the uterus of transgenic mouse causes downregulation of TGF-beta receptors and delays the blastocyst-attachment reaction J Mole Endocrinol 18 243–257 Occurrence Handle10.1677/jme.0.0180243 JJ Dore JE Wilkinson JD Godkin (1996) ArticleTitleOvien endometrial expression of transforming growth factor beta isoforms during the peri-implantation period Biol Reprod 54 1080–1087 Occurrence Handle8722629 J Fujimoto S Ichigo M Hori T Tamaya (1996) ArticleTitleExpression of E-cadherin, alpha- and beta-cadherin mRNAs in ovarian endometriosis Eur J Obstet Gynecol Reprod Biol 67 179–183 Occurrence Handle10.1016/0301-2115(96)02432-3 Occurrence Handle8841809 F Furukawa K Fujii Y Horiguchi N Matsuyoshi M Fujita K Toda S Imamura H Wakita S Shirahama M Takigawa (1997) ArticleTitleRole of E- and P-cadherin in the human skin Microsc Res Tech 38 343–352 Occurrence Handle10.1002/(SICI)1097-0029(19970815)38:43.0.CO;2-K Occurrence Handle9297684 R Gaetje S Kotzian G Herrmann R Baumann A Starzinski-Powitz (1997) ArticleTitleNonmalignant epithelial cells, potentially invasive in human endometriosis, lack the tumor suppressor molecule E-cadherin Am J Pathol 150 461–467 Occurrence Handle9033262 Y Gu WS Branham DM Sheehan PJ Webb CL Moland RD Streck (1999) ArticleTitleTissue-specific expression of messenger ribonucleic acids for insulin-like growth factors and insulin-like growth factor-binding proteins during perinatal development of the rat uterus Biol Reprod 60 1172–1182 Occurrence Handle10208980 GM Hansch C Wagner A Burger W Dong G Staehler M Stoeck (1995) ArticleTitleMatrix protein synthesis by glomerular mesangial cells in culture: effects of transforming growth factor beta (TGF beta) and platelet-derived growth factor (PDGF) on fibronectin and collagen type IV mRNA J Cell Physiol 163 451–457 Occurrence Handle10.1002/jcp.1041630304 Occurrence Handle7775588 RG Hardy RM Brown SJ Miller C Tselepis DG Morton JA Jankowski DS Sanders (2002) ArticleTitleTransient P-cadherin expression in radiation proctitis: a model of mucosal injury and repair J Pathol 197 194–200 Occurrence Handle10.1002/path.1092 Occurrence Handle12015743 RG Hardy C Tselepis J Hoyland Y Wallis TP Pretlow I Talbot DS Sanders G Matthews D Morton JA Jankowski (2002) ArticleTitleAberrant P-cadherin expression is an early event in hyperplastic and dysplastic transformation in the colon Gut 50 513–519 Occurrence Handle10.1136/gut.50.4.513 Occurrence Handle11889072 D Li W Zhang AA Sahin WN Hittelman (1999) ArticleTitleDNA adducts in normal tissue adjacent to breast cancer: a review Cancer Detect Prev 23 454–462 Occurrence Handle10.1046/j.1525-1500.1999.99059.x Occurrence Handle10571655 G Loverro E Maiorano A Napoli L Selvaggi E Marra E Perlino (2001) ArticleTitleTransforming growth factor-beta 1 and insulin-like growth factor-I expression in ovarian endometriotic cysts: a preliminary study Int J Mole Med 7 423–429 WJ Murdoch (2003) ArticleTitleMetaplastic potential of p53 down-regulation in ovarian surface epithelial cells affected by ovulation Cancer Lett 191 75–81 Occurrence Handle10.1016/S0304-3835(02)00623-7 Occurrence Handle12609712 A Nunomura (1999) J Neurosci 19 1959–1968 Occurrence Handle10066249 AR Parker RN O’Meally DH Oliver L Hua WG Nelson TL DeWeese JR Eshleman (2002) ArticleTitle8-Hydroxyguanosine repair is defective in some microsatellite stable colorectal cancer cells Cancer Res 62 7230–7233 Occurrence Handle12499263 R Paul CM Ewing DF Jarrard WB Isaacs (1997) ArticleTitleThe cadherin cell–cell adhesion pathway in prostate cancer progression Br J Urol 79 37–43 Occurrence Handle9088271 V Polli C Bulletti A Galassi A Borini PM Ciotti R Seracchioli S Alfieri C Flamigni (1996) ArticleTitleTransforming growth factor-beta 1 in the human endometrium Gynecol Endocrinol 10 297–302 Occurrence Handle8915658 Robboy S, Anderson M, Russell P (Eds.) (2002) Pathology of the Female Reproductive Tract, Churchill Livingston, Sydney. Rosai J (Ed.) (1996) Ackerman’s Surgical Pathology, Mosby, New York. DS Sanders I Perry R Hardy J Jankowski (2000) ArticleTitleAberrant P-cadherin expression is a feature of clonal expansion in the gastrointestinal tract associated with repair and neoplasia J Pathol 190 526–530 Occurrence Handle10.1002/(SICI)1096-9896(200004)190:53.0.CO;2-9 Occurrence Handle10727977 M Sbracia E Zupi P Alo C Manna D Marconi F Scarpellini JA Grasso U Di Tondo C Romanini (1997) ArticleTitleDifferential expression of IGF-I and IGF-II in eutopic and ectopic endometria of women with endometriosis and in women without endometriosis Am J Reprod Immunol 37 326–329 Occurrence Handle9161641 D Scott A Sanders I Perry R Hardy J Jankowski (2000) ArticleTitleAberrant P-cadherin expression is a feature of clonal expansion in the gastrointestinal tract associated with repair and neoplasia J Pathol 190 526–530 Occurrence Handle10.1002/(SICI)1096-9896(200004)190:53.0.CO;2-9 Occurrence Handle10727977 S Scotti PA Regidor AE Schindler E Winterhager (2000) ArticleTitleReduced proliferation and cell adhesion in endometriosis Mole Hum Reprod 6 610–617 Occurrence Handle10.1093/molehr/6.7.610 M Slater (1999) J Histotechnol 22 17–21 M Slater JA Barden CR Murphy (2000) Histochem J 32 365–372 Occurrence Handle10.1023/A:1004017714702 Occurrence Handle10943851 M Slater CR Murphy (1999a) ArticleTitleChondroitin sulphate and heparin sulfate proteoglycan are sequentially expressed in the uterine extracellular matrix during early pregnancy in the rat Matrix Biol 18 125–131 Occurrence Handle10.1016/S0945-053X(99)00006-2 M Slater CR Murphy (1999b) ArticleTitleDifferential expression of insulin-like growth factors in the uterine epithelium and extracellular matrix during early pregnancy Matrix Biol 18 579–584 Occurrence Handle10.1016/S0945-053X(99)00051-7 M Slater CR Murphy (1999c) ArticleTitlePremature implantation may be prevented by an inhibitory system regulated by epidermal growth factor Acta Histochem 101 121–126 M Slater CR Murphy (1999d) ArticleTitleThrombospondin is sequentially expressed and then de-expressed during early pregnancy in the rat uterus Histochem J 31 471–475 Occurrence Handle10.1023/A:1003760026681 M Slater CR Murphy (2000) ArticleTitleTransforming growth factors alpha and beta-1 are co-expressed in the uterine epithelium during early pregnancy Cell Tissue Res 300 315–320 RA Stern R Dash B R (2001) J Gynecol Pathol 20 133–139 Occurrence Handle10.1097/00004347-200104000-00004 M Takeichi (1993) ArticleTitleCadherins in cancer: implications for invasion and metastasis Curr Opin Cell Biol 5 806–811 Occurrence Handle10.1016/0955-0674(93)90029-P Occurrence Handle8240824 M Kleffens Particlevan C Groffen DJ Lindenberghkortleve JW Vanneck S Gonzalezparra N Dits EC Zwarthoff SLS Drop (1998) ArticleTitleThe IGF system during fetal–placental development of the mouse Mole Cell Endocrinol 140 129–135 Occurrence Handle10.1016/S0303-7207(98)00041-0 K Vleminckx L Vakaet M Mareel (1994) ArticleTitleLoss of membranous E-cadherin expression in pancreatic cancer: correlation with lymph node metastasis, high grade, and advanced stage J Pathol 174 243–248 Occurrence Handle10.1002/path.1711740403 Occurrence Handle7884585 YZ Wang YC Wong (1998) ArticleTitleSex hormone-induced prostatic carcinogenesis in the noble rat: the role of insulin-like growth factor I (IGF-I) and vascular endothelial growth factor (VEGF) in the development of prostate cancer Prostate 35 165–177 Occurrence Handle10.1002/(SICI)1097-0045(19980515)35:33.0.CO;2-G Occurrence Handle9582085 DY Xing W Tan N Song DX Lin (2001) ArticleTitleSer326Cys polymorphism in hOGG1 gene and risk of esophageal cancer in a Chinese population Int J Cancer 95 140–143 Occurrence Handle10.1002/1097-0215(20010520)95:33.0.CO;2-2 Occurrence Handle11307145 X Zhang GM Kidder AJ Watson GA Schultz DT Armstrong (1994) ArticleTitlePossible roles of insulin and insulin-like growth factors in rat preimplantation development: investigation of gene expression by reverse transcription-polymerase chain reaction J Reprod Fertil 100 375–380 Occurrence Handle7517453 Author information Authors and Affiliations Corresponding author Rights and permissions About this article Cite this article Slater, M., Quagliotto, G., Cooper, M. et al. Endometriotic Cells Exhibit Metaplastic Change and Oxidative DNA Damage as Well as Decreased Function, Compared to Normal Endometrium. J Mol Hist 36, 257–263 (2005). https://doi.org/10.1007/s10735-005-3802-9 Received: Accepted: Issue date: DOI: https://doi.org/10.1007/s10735-005-3802-9