Partial purification and amino acid sequence analysis of endometriosis protein-II (ENDO-II) reveals homology with tissue inhibitor of metalloproteinases-1 (TIMP-1)
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Endometriosis protein-II (ENDO-II) was partially purified from rat endometriotic tissue, and amino acid sequencing revealed homology with tissue inhibitor of metalloproteinases-1 (TIMP-1).
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Abstract
De novo synthesized endometriosis protein-II (ENDO-II; M(r) 28,000 to 32,000; pI 7.0 to 9.0) was partially purified from rat endometriotic tissue culture media using affinity chromatography and two-dimensional SDS-PAGE. The protein was electrophoretically transferred to polyvinyl difluoride membranes which were stained with Coomassie blue R-250. The stained protein corresponding to ENDO-II (M(r) 28,000 to 32,000; pI 7.0 to 9.0) was cut from the membranes for amino acid sequencing. Partial amino acid sequence was determined by automated Edman degradation using a gas phase sequencer and phenylthiohydantoin analyzer. Sequence analysis of ENDO-II yielded 25 residues: C S C A P T H P Q T A F C N S D L V I R K F M G. Comparison to sequence databanks demonstrated significant homology with rat (100%) and human (84%) tissue inhibitor of metalloproteinases-1 (TIMP-1). Western blot analysis using a TIMP-1 antibody confirmed amino acid sequence analysis. In conclusion, ENDO-II shares sequence homology with TIMP-1 and cross-reactivity with TIMP-1 antibody and subsequently identifies production of TIMP-1 by endometriotic tissues. The synthesis and secretion of TIMP-1 by endometriosis may derange the normal proteolytic milieu of the peritoneal cavity and contribute to the etiology and underlying physiological sequelae associated with the disease endometriosis.
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Cited by (32)
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- Proteomic analysis of peritoneal fluid identified COMP and TGFBI as new candidate biomarkers for endometriosis 2021
- Cervical mucus proteome in endometriosis 2017
- Endometriosis 2015
- Cellular and molecular basis for endometriosis-associated infertility 2012
- TIMP1 contributes to ovarian anomalies in both an MMP-dependent and -independent manner in a rat model 2011
- Cinética celular na endometriose profunda infiltrativa de reto-sigmoide: estudo anátomo-clínico 2011
- Post-translational modifications and protein-specific isoforms in endometriosis revealed by 2D DIGE 2010
- Reduced fecundity in female rats with surgically induced endometriosis and in their daughters: a potential role for tissue inhibitors of metalloproteinase 1 2008
- Use of proteomic analysis of endometriosis to identify different protein expression in patients with endometriosis versus normal controls 2006
- Aromatase in endometriosis and uterine leiomyomata 2005
- mRNA analysis of several components of the plasminogen activator and matrix metalloproteinase systems in endometriosis using a real-time quantitative RT-PCR assay 2004
- Expression of several components of the plasminogen activator and matrix metalloproteinase systems in endometriosis 2003
- Mechanisms of excessive estrogen formation in endometriosis 2002
- Diagnostik und Therapie der Endometriose 2002
- Estrogen production and metabolism in endometriosis 2002
- Using rats as a research model for the study of endometriosis 2002
- Paracrine regulation of matrix metalloproteinase expression in endometriosis 2002
- Differential regulation of matrix metalloproteinase-3 gene expression in endometriotic lesions compared with endometrium 2001
- Endometrial anomalies in women with endometriosis 2001
- Angiogenesis in Endometriosis 2001
- Aromatase as a therapeutic target in endometriosis 2000
- Estrogen biosynthesis in endometriosis: molecular basis and clinical relevance 2000
- Estrogen production in endometriosis and use of aromatase inhibitors to treat endometriosis 1999
- Peritoneal endometriotic lesions differentially express a haptoglobin-like gene 1999
- Aromatase: a key molecule in the pathophysiology of endometriosis and a therapeutic target 1999
- Novel implications in the development of endometriosis: biphasic effect of macrophage activation on peritoneal tissue expression of tissue inhibitor of metalloproteinase-1 1998
- Tissue inhibitor of metalloproteinase-1 concentrations are attenuated in peritoneal fluid and sera of women with endometriosis and restored in sera by gonadotropin-releasing hormone agonist therapy 1998
- Deficient 17beta-hydroxysteroid dehydrogenase type 2 expression in endometriosis: failure to metabolize 17beta-estradiol 1998
- Pathogenesis of endometriosis 1997
- Basic research in endometriosis 1997
- Expression of interstitial collagenase (matrix metalloproteinase-1) is related to the activity of human endometriotic lesions 1997
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