Constitutive and tumor necrosis factor-alpha-stimulated activation of nuclear factor-kappaB in immortalized endometriotic cells and their suppression by trichostatin A

Yan Wu, Wu Y, Starzinski-Powitz A, Anna Starzinski-Powitz, Anna Starzinski‐Powitz, Sun‐Wei Guo, Sun-Wei Guo, Guo SW
Gynecologic and obstetric investigation · 2010 · vol. 70(1) , pp. 23–33 · doi:10.1159/000279324 · PMID:20130413 · W2033505348
Lab / animal OA: closed CC0 ⤵ 16 in-corpus citations
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This study found that NF-kappaB is constitutively activated in endometriotic cells, and this activation is suppressed by trichostatin A, which also affects p65 phosphorylation and translocation.

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Abstract

OBJECTIVE(S): To determine whether nuclear factor-kappaB (NF-kappaB) is constitutively and tumor necrosis factor (TNF)-dependently activated in endometriotic cells, whether trichostatin A (TSA) can suppress NF-kappaB activation and suppress TRAF2/6 and TAK1, and whether TSA and caffeic acid phenyl ester can suppress constitutive and H(2)O(2)-stimulated proliferation of endometriotic cells. METHODS: Two endometriotic cell lines and an endometrial stromal cell line were used as an in vitro model. Electrophoretic mobility shift analysis was used to determine NF-kappaB activation and possible suppression by TSA. Western blot analysis was used to determine whether TSA suppresses phosphorylation of IkappaBalpha, phosphorylation of p65 in the cytoplasm and nuclear translocation, and the expression of TRAF2/6 and TAK1. RESULTS: NF-kappaB was constitutively activated in endometriotic cells, but only minimally in endometrial cells. TNFalpha stimulation activated NF-kappaB through induction of IkappaB phosphorylation, but the activation can be suppressed by TSA. TSA also attenuated constitutive and TNF-dependent p65 phosphorylation and nuclear translocation in endometriotic cells. TRAF2, TRAF6 and TAK1 were constitutively activated and were unaffected by TSA treatment. CONCLUSIONS: NF-kappaB activation may play a critical role in the pathogenesis in endometriosis. Targeting NF-kappaB with histone deacetylase inhibitors or other compounds might hold promise as novel therapeutics for endometriosis.

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Condition tags

endometriosis

MeSH descriptors

Endometrium Hydroxamic Acids Stromal Cells TNF Receptor-Associated Factor 2 TNF Receptor-Associated Factor 6 Transcription Factor RelA Caffeic Acids Caffeic Acids Caffeic Acids Cell Division Cell Division Cell Division Cell Division Cell Line, Transformed Cell Line, Transformed Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis

Citation neighborhood (2-hop)

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. Outer rings show 2-hop neighbours — papers reached through the immediate citers/citees. [ collapse to 1-hop ]

References (60)

Cited by (18)

Source provenance

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