RU486-induced growth inhibition of human endometrial cells

Ana A. Murphy, A A Murphy, Murphy AA, Zhou MH, M H Zhou, Mimi H Zhou, Srividya Malkapuram, S Malkapuram, Malkapuram S, Nalini Santanam, N Santanam, Sampath Parthasarathy, Parthasarathy S, S Parthasarathy, Sidell N, N Sidell, Neil Sidell
Fertility and Sterility · 2000 · vol. 74(5) , pp. 1014–1019 · doi:10.1016/s0015-0282(00)01606-x · PMID:11056251 · W2025109517
article OA: bronze CC0 ⤵ 25 in-corpus citations
🔓 Open OA copy View on OpenAlex View on PubMed View at publisher
⚙ AI-generated summary by claude@2026-07, 2026-07-03 ⓘ

This study investigated the effects of RU486 on human endometrial cells and found that it inhibited their growth.

One-sentence paraphrase of the abstract; not a substitute for reading it. No clinical advice. How this works

Abstract

ObjectiveTo determine the direct action of RU486 on endometrial cell proliferation and to differentiate whether the antioxidant or the antiprogesterone property of RU486 is predominately responsible for its effect on cell growth.DesignIn vitro study comparing the effects of RU486 (antiprogesterone and antioxidant), reduced RU486 (antioxidant), ZK112,993 (antiprogesterone), and lazaroid U74,500A (antioxidant) on endometrial cell growth. The human endometrial cell line EM42 was used in transient transfection assays to confirm the relative antiprogesterone potency of the various compounds.SettingAcademic medical centerPatient(s)Women presenting with pelvic pain or infertility and diagnosed with endometriosis at time of surgery or women desiring tubal ligation with a normal pelvis (controls).Intervention(s)Endometrial cell cultures were treated with RU486, reduced RU486, lazaroid U74,500A, and ZK112,993.Main outcome measure(s)Tritiated thymidine incorporation was used to assess cell growth. Inhibition of progesterone induction of transiently transfected reporter plasmids was used to measure antiprogesterone activity of compounds studied.Result(s)RU486 reduced cell growth in a dose-dependent fashion of the endometrial cell lines EM42 and RL95-2 and of endometrial and endometriosis cells from primary culture. After being reduced, RU486 lost most of its antiprogesterone activity but retained its antiproliferative properties. ZK112,993 was similar in potency to RU486 as a progesterone antagonist but did not significantly modify endometrial cell growth. Lazaroid U74,500A was devoid of antiprogesterone activity but was shown to be a potent antiproliferative agent.Conclusion(s)RU486 has a direct inhibitory effect on human endometrial cell growth. This activity appears to be at least partly mediated through its antioxidant property.

My notes (saved in your browser only)

MeSH descriptors

Antioxidants Endometrium Hormone Antagonists Mifepristone Mifepristone Antioxidants Cell Division Cell Division Cell Line Endometrium Endometrium Female Hormone Antagonists Humans Mifepristone Pregnatrienes Pregnatrienes

Citation neighborhood

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

References (19)

Cited by (25)

Source provenance

europepmc
last seen: 2026-07-27T06:15:28.040536+00:00
openalex
last seen: 2026-05-11T06:38:17.545654+00:00
pubmed
last seen: 2026-05-13T22:13:30.513821+00:00
License: CC0 · commercial use OK