The roles of thioredoxin and thioredoxin-binding protein-2 in endometriosis

Seok Kyo Seo, Hyuk In Yang, Hyo In Yang, K. E. Lee, Kyung Eun Lee, Hye Yeon Kim, Hyeyoung Kim, Sihyun Cho, Young Sik Choi, Byung Seok Lee
Human Reproduction · 2010 · vol. 25(5) , pp. 1251–1258 · doi:10.1093/humrep/deq027 · PMID:20172870 · W2109968736
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⚙ AI-generated summary by claude@2026-06, 2026-06-12 ⓘ

Endometrial TBP-2 mRNA levels were lower and the TRX to TBP-2 ratio was higher in endometriosis patients compared to controls, suggesting aberrant expression may be linked to disease.

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This Human Reproduction study examined whether the redox regulator thioredoxin (TRX) and its negative regulator thioredoxin-binding protein-2 (TBP-2) are dysregulated in endometriosis, using 35 women with histologically confirmed endometriosis and 31 without the condition. TRX and TBP-2 mRNA levels were measured in endometrium by real-time PCR, protein localization was assessed by immunohistochemistry, and serum/peritoneal fluid levels were quantified by ELISA. Endometriosis patients showed no endometrial TRX mRNA difference versus controls, but had lower TBP-2 mRNA and a higher TRX:TBP-2 mRNA ratio, with this ratio particularly elevated in late secretory and menstrual phases; immunohistochemistry similarly indicated decreased TBP-2 immunoreactivity. The paper reports no significant serum or peritoneal fluid differences and notes that TRX and TBP-2 mRNA levels did not correlate in endometriosis patients, unlike a positive correlation observed in controls. This paper is centrally about endometriosis — it investigates TRX and TBP-2 dysregulation in endometrial tissue as a potential part of endometriosis pathophysiology.

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Abstract

BACKGROUND: Oxidative stress is considered to be involved in the establishment and development of endometriosis. Thioredoxin (TRX) is an endogenous redox regulator that protects cells against oxidative stress, and TRX-binding protein-2 (TBP-2) is a negative regulator of TRX in the biological function and expression. The aim of this study was to investigate the roles of TRX and TBP-2 in the pathophysiology of endometriosis. METHODS: A total of 35 patients with histologically confirmed endometriosis and 31 patients without endometriosis participated in this study. Real-time polymerase chain reaction was used to quantify TRX and TBP-2 mRNA levels, and immunohistochemistry (IHC) was used to assess TRX and TBP-2 protein localization in the endometrium. Serum and peritoneal fluid levels of TRX and TBP-2 were measured using a specific commercial ELISA. RESULTS: There were no significant differences in TRX mRNA levels in the endometrium of patients with endometriosis and the control groups. However, TBP-2 mRNA levels in the endometrium were lower, and the TRX to TBP-2 ratio was higher in patients with endometriosis than in the control group. In particular, the TRX to TBP-2 ratio was significantly higher during late secretory and menstrual phase in patients with endometriosis compared with the control group. IHC studies also showed the decreased TBP-2 immunoreactivity in patients with endometriosis compared with the control group. There was no correlation between TRX and TBP-2 mRNA levels in patients with endometriosis, whereas TRX mRNA levels were positively correlated with TBP-2 mRNA levels in the control group. There were no significant differences between the two groups in TRX and TBP-2 levels in serum or peritoneal fluid. CONCLUSIONS: Aberrant expression of TRX and TBP-2 in the endometrium may be associated with the establishment of endometriosis.
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Methods

A total of 35 patients with histologically confirmed endometriosis and 31 patients without endometriosis participated in this study. Real-time polymerase chain reaction was used to quantify TRX and TBP-2 mRNA levels, and immunohistochemistry (IHC) was used to assess TRX and TBP-2 protein localization in the endometrium. Serum and peritoneal fluid levels of TRX and TBP-2 were measured using a specific commercial ELISA.

Results

There were no significant differences in TRX mRNA levels in the endometrium of patients with endometriosis and the control groups. However, TBP-2 mRNA levels in the endometrium were lower, and the TRX to TBP-2 ratio was higher in patients with endometriosis than in the control group. In particular, the TRX to TBP-2 ratio was significantly higher during late secretory and menstrual phase in patients with endometriosis compared with the control group. IHC studies also showed the decreased TBP-2 immunoreactivity in patients with endometriosis compared with the control group. There was no correlation between TRX and TBP-2 mRNA levels in patients with endometriosis, whereas TRX mRNA levels were positively correlated with TBP-2 mRNA levels in the control group. There were no significant differences between the two groups in TRX and TBP-2 levels in serum or peritoneal fluid.

Conclusions

Aberrant expression of TRX and TBP-2 in the endometrium may be associated with the establishment of endometriosis - Files in This Item: - T201001944.pdf Download - Appears in Collections: - 1. College of Medicine (의과대학) > Dept. of Obstetrics and Gynecology (산부인과학교실) > 1. Journal Papers - Yonsei Authors - Kim, Hye Yeon(김혜연) Seo, Seok Kyo(서석교) https://orcid.org/0000-0003-3404-0484 Yang, Hyo In(양효인) Lee, Kyung Eun(이경은) Lee, Byung Seok(이병석) https://orcid.org/0000-0001-6001-2079 Cho, Si Hyun(조시현) https://orcid.org/0000-0003-2718-6645 Choi, Young Sik(최영식) https://orcid.org/0000-0002-1157-4822 Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

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Condition tags

endometriosis

MeSH descriptors

Carrier Proteins Carrier Proteins Endometriosis Endometriosis Thioredoxins Thioredoxins Adult Ascitic Fluid Ascitic Fluid Base Sequence Carrier Proteins Carrier Proteins Case-Control Studies DNA Primers DNA Primers Endometriosis Endometriosis Endometrium Endometrium Female

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