{"paper_id":"cf346378-31ab-4ddb-bdab-43fa81c9cc3d","body_text":"YUHASpace Repository\n570\n818\nCited 0 times in\nCited 18 times in\nThe roles of thioredoxin and thioredoxin-binding protein-2 in endometriosis\n- Authors\n- Seok Kyo Seo ; Hyo In Yang ; Kyung Eun Lee ; Hye Yeon Kim ; SiHyun Cho ; Young Sik Choi ; Byung Seok Lee\n- Citation\n- HUMAN REPRODUCTION, Vol.25(5) : 1251-1258, 2010\n- Journal Title\n- HUMAN REPRODUCTION\n- ISSN\n- 0268-1161\n- Issue Date\n- 2010\n- MeSH\n- Adult ; Ascitic Fluid/metabolism ; Base Sequence ; Carrier Proteins/blood ; Carrier Proteins/genetics* ; Carrier Proteins/metabolism* ; Case-Control Studies ; DNA Primers/genetics ; Endometriosis/etiology ; Endometriosis/genetics* ; Endometriosis/metabolism* ; Endometrium/metabolism ; Female ; Humans ; Immunohistochemistry ; Middle Aged ; Polymerase Chain Reaction ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Thioredoxins/blood ; Thioredoxins/genetics* ; Thioredoxins/metabolism* ; Young Adult\n- Abstract\n- BACKGROUND: Oxidative stress is considered to be involved in the establishment and development of endometriosis. Thioredoxin (TRX) is an endogenous redox regulator that protects cells against oxidative stress, and TRX-binding protein-2 (TBP-2) is a negative regulator of TRX in the biological function and expression. The aim of this study was to investigate the roles of TRX and TBP-2 in the pathophysiology of endometriosis.\nMETHODS: A total of 35 patients with histologically confirmed endometriosis and 31 patients without endometriosis participated in this study. Real-time polymerase chain reaction was used to quantify TRX and TBP-2 mRNA levels, and immunohistochemistry (IHC) was used to assess TRX and TBP-2 protein localization in the endometrium. Serum and peritoneal fluid levels of TRX and TBP-2 were measured using a specific commercial ELISA.\nRESULTS: There were no significant differences in TRX mRNA levels in the endometrium of patients with endometriosis and the control groups. However, TBP-2 mRNA levels in the endometrium were lower, and the TRX to TBP-2 ratio was higher in patients with endometriosis than in the control group. In particular, the TRX to TBP-2 ratio was significantly higher during late secretory and menstrual phase in patients with endometriosis compared with the control group. IHC studies also showed the decreased TBP-2 immunoreactivity in patients with endometriosis compared with the control group. There was no correlation between TRX and TBP-2 mRNA levels in patients with endometriosis, whereas TRX mRNA levels were positively correlated with TBP-2 mRNA levels in the control group. There were no significant differences between the two groups in TRX and TBP-2 levels in serum or peritoneal fluid.\nCONCLUSIONS: Aberrant expression of TRX and TBP-2 in the endometrium may be associated with the establishment of endometriosis\n- Files in This Item:\n- T201001944.pdf Download\n- Appears in Collections:\n- 1. College of Medicine (의과대학) > Dept. of Obstetrics and Gynecology (산부인과학교실) > 1. Journal Papers\n- Yonsei Authors\n-\nKim, Hye Yeon(김혜연)\nSeo, Seok Kyo(서석교) https://orcid.org/0000-0003-3404-0484\nYang, Hyo In(양효인)\nLee, Kyung Eun(이경은)\nLee, Byung Seok(이병석) https://orcid.org/0000-0001-6001-2079\nCho, Si Hyun(조시현) https://orcid.org/0000-0003-2718-6645\nChoi, Young Sik(최영식) https://orcid.org/0000-0002-1157-4822\nItems in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.","source_license":"CC0","license_restricted":false}