Tokishakuyakusan, a Kampo medicine, attenuates endometriosis-like lesions and hyperalgesia in murine with endometriosis-like symptoms

Kei Nagira, Nagira K, Fuminori Taniguchi, Nakamura K, Kazuomi Nakamura, Yohei Tokita, Naoko Tsuchiya, Tsuchiya N, Yin Mon Khine, Khine YM, Tasuku Harada, Harada T
American journal of reproductive immunology (New York, N.Y. : 1989) · 2019 · vol. 82(5) , pp. e13182 · doi:10.1111/aji.13182 · PMID:31446641 · W2969302534
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Tokishakuyakusan treatment in mice with an endometriosis model significantly reduced lesion formation and hyperalgesia, with decreased IL-33 expression in lesions.

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Abstract

PROBLEM: How are the effects of Tokishakuyakusan (TSS), a traditional Japanese medicine (Kampo) on murine endometriosis model? METHODS: BALB/c mice were used for making the murine endometriosis model. Homogeneous uterus was surgically implanted with lipopolysaccharide (LPS) in peritoneal cavity. We administered 2 weeks of TSS (1.0 g/kg) orally. Upon treatment completion, we performed the hot plate test for all mice and collected blood samples before sacrifice. Then, the endometriosis-like lesions and uteri in the abdominal cavity were harvested. Concentrations of several cytokines in sera and cyst fluids were measured using Bio-Plex Suspension Array System. IL-33 localization was determined by immunohistochemistry. Gene expression of inflammatory cytokines in the endometriosis-like lesions or the eutopic endometrium was evaluated by real-time RT-PCR. RESULTS: After 14 days of TSS treatment, the numbers of endometriosis-like cysts and cyst weight were significantly decreased. In TSS-treated mice, the latency against heat stimuli was extended. Inflammatory cytokine concentrations in sera were not changed by TSS treatment. TSS intake decreased IL-33 mRNA expression in endometriosis-like lesions and led to the tendency of attenuation of the elevated IL-33 synthesis in the cyst fluids of lesions. CONCLUSION: These results suggest the TSS ameliorated the hyperalgesia and lesion formation on the LPS-accelerated endometriosis-like model. TSS represents a possible ideal target of novel therapeutics for endometriosis patients with dysmenorrhea.

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Condition tags

endometriosisdysmenorrhea

MeSH descriptors

Drugs, Chinese Herbal Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Hyperalgesia Hyperalgesia Hyperalgesia Hyperalgesia Hyperalgesia Medicine, Kampo Medicine, Kampo Animals Animals Disease Models, Animal Disease Models, Animal Drugs, Chinese Herbal Drugs, Chinese Herbal Female

Citation neighborhood (2-hop)

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. Outer rings show 2-hop neighbours — papers reached through the immediate citers/citees. [ collapse to 1-hop ]

References (38)

Cited by (7)

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