Effect of Genistein on Endometriosis Lesion, Matrix Metalloproteinase-2 and -9 Level of Endometriosis: In silico and In vivo Study

Maharani Maharani, Endang Sri Wahyuni, Sutrisno Sutrisno
In: Journal of Clinical and Molecular Endocrinology · 2016 · vol. 1(1) · W2501404495
article OA: closed CC0 ⤵ 3 in-corpus citations Metadata only
View on OpenAlex
⚙ AI-generated summary by claude@2026-06, 2026-06-08 ⓘ

This study found that genistein treatment in a mouse model of endometriosis reduced lesion size and significantly attenuated the elevated levels of MMP-2 and MMP-9.

One-sentence paraphrase of the abstract; not a substitute for reading it. No clinical advice. How this works

Abstract

This in silico and in vivo investigation were to know the effect of genistein on alteration of MMPs in a mice model of endometriosis. Forty female mice (Mus musculus) were divided into eight groups (n=5 each), including the control (untreated) group, endometriosis group, and the endometriosis (EM) groups were given various doses of genistein (at dose of 50; 100; 200; 300; 400; 500 mg/day). In silico analysis was performed using Open Babel, 3D DART webserver, HEX software, and NUCPLOT software. Analysis of MMP-2 and MMP-9 level were done by enzyme linked immuno sorbent assay (ELISA) technically. In the absence of genistein, ΣE total required for the interaction between NF-?B with MMP-2 or MMP-9 gene promoter were increased in genistein treatment (-2393.71 kJ/mol and -2393.86 kJ/mol) compared with no genistein treatment (-2415.62 kJ/mol and -2457.00 kJ/mol). In endometriosis group we were founded the massive endometriosis lesion and hyper vascularization. All doses the treatment with genistein reduces the size of endometriosis lesion. The level of MMP-2 and MMP-9 was significantly higher in the EM group compared to the untreated control group (P<0.05). This increase in MMP-2 was significantly (P<0.05) attenuated by all doses of genistein. These increased levels of MMP-9 in the EM group were significantly reduced by 100 mg/day administration of genistein. In conclusion, genistein prohibits the increase in MMP-2 and MMP-9 in a mice model of endometriosis. Therefore, this may provide a natural therapy for attenuating the alteration of MMPs found in endometriosis disease.

My notes (saved in your browser only)

Condition tags

endometriosis

Citation neighborhood

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

References (33)

Cited by (3)

Source provenance

openalex
last seen: 2026-06-10T17:14:06.276822+00:00
License: CC0 · commercial use OK