Establishment of Immortalized Human Endometriotic Stromal Cell Line from Ectopic Lesion of a Patient with Endometriosis

Daryeon Son, Hahyun Park, Garam An, Sunwoo Park, Sung Soo Park, Dong Won Hwang, Soo Jin Park, Hee Seung Kim, Whasun Lim, Seungkwon You, Gwonhwa Song
Reproductive sciences (Thousand Oaks, Calif.) · 2023 · vol. 30(9) , pp. 2703–2714 · doi:10.1007/s43032-023-01225-9 · PMID:37067725 · W4366083650
article OA: closed CC0 ⤵ 4 in-corpus citations
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⚙ AI-generated summary by claude@2026-06+body, 2026-06-07 ⓘ

This study established immortalized human endometriotic stromal cell lines (ihOESCs) via hTERT transfection, maintaining phenotypic and functional properties for extended study of endometriosis pathogenesis.

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⚙ AI-generated deep summary by claude@2026-06, 2026-06-07 ⓘ

The paper aimed to develop a durable in vitro model to study molecular mechanisms of endometriosis by deriving primary human ovarian endometriotic stromal cells (hOESCs) from a reproductive-age patient and then immortalizing them. Primary cells were immortalized with human telomerase reverse transcriptase (hTERT), producing immortalized ihOESC lines that maintained proliferative capacity through passage without mutagenesis during senescence, while preserving morphology and karyotype relative to the primary cells. After decidual stimuli and inflammatory challenge, both primary and immortalized cells showed expression of decidualization markers and proinflammatory cytokines, and the immortalized cells retained vimentin-positive/E-cadherin-negative phenotypes. A major limitation stated implicitly by the design is that the work is based on cells from a single patient-derived line rather than broad patient sampling. This paper is centrally about endometriosis — establishing an immortalized human ovarian endometriotic stromal cell line derived from ectopic lesions to study disease mechanisms.

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Abstract

Endometriosis is an estrogen-dependent inflammatory disease characterized by the growth of endometrial-like tissues containing endometrial stromal cells and glandular epithelium outside the uterine cavity. An insufficient response to progesterone contributes to disease progression and systemic inflammation during the pathogenesis of endometriosis. Patients with endometriosis usually experience painful symptoms, dysmenorrhea, and infertility, which contribute to a significant reduction in their quality of life. To determine the possible molecular mechanisms of endometriosis and explore novel therapeutic targets, we derived primary human ovarian endometriotic stromal cells (hOESCs) from a patient of reproductive age with ovarian endometriosis. In this study, we successfully established immortalized human ovarian endometriotic stromal cell lines (ihOESCs) using primary stromal cells obtained from endometriotic lesions to overcome short lifespan and growth inhibition. Immortalization of hOESCs with human telomerase reverse transcriptase (hTERT) transfection led to cells that maintained a proliferative state under passage culture conditions without mutagenesis during cellular senescence. The morphology and karyotype of ihOESCs were unchanged compared with those of hOESCs. Moreover, ihOESCs were continuously positive for vimentin and negative for E-cadherin expression. Following decidual stimuli and inflammatory responses, both hOESCs and ihOESCs sensitively express decidualization markers and proinflammatory cytokines. Collectively, we characterized ihOESCs to maintain their phenotypic and functional properties with a longer lifespan and normal physiological responses than those of hOESCs. These immortalized cells could aid in a detailed understanding of the pathological mechanisms of endometriosis.

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Condition tags

endometriosis

MeSH descriptors

Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis

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