Annelyn Torres-Reverón (5650028)

IntroductionWe have previously shown that Environmental Enrichment (EE), a multi-modal psychosocial intervention consisting of increased social interaction, novelty, and open spaces, improved disease presentation, anxiety, and immune-relate…

IntroductionWe have previously shown that Environmental Enrichment (EE), a multi-modal psychosocial intervention consisting of increased social interaction, novelty, and open spaces, improved disease presentation, anxiety, and immune-relate…

IntroductionWe have previously shown that Environmental Enrichment (EE), a multi-modal psychosocial intervention consisting of increased social interaction, novelty, and open spaces, improved disease presentation, anxiety, and immune-relate…

IntroductionWe have previously shown that Environmental Enrichment (EE), a multi-modal psychosocial intervention consisting of increased social interaction, novelty, and open spaces, improved disease presentation, anxiety, and immune-relate…

The protocol for tissue collection used in the current manuscript is similar to the one previously published [22]. Two separate cohorts of rats were used: one that received endometriosis or sham surgery and allowed to progress until 7 days …

Endometriosis vesicle measurements in animals for which adrenal receptors were analyzed.

Total adrenal weight (A) and mRNA quantification of the mineralocorticoid receptor (B), glucocorticoid receptor (C), corticotrophin releasing hormone receptor type 1 (CRHR1; D) and type 2 (CRHR2; E). N = 8 in each group. For this and all su…

Total adrenal weight normalized by rat body weight (A), adrenocorticotropic hormone (ACTH) concentration in serum (B) and corticosterone concentration in serum (C). * represents p< 0.05 on an ANOVA test.

mRNA quantification of the mineralocorticoid receptor (MCR2; A), glucocorticoid receptor (GR; B). Ratio of glucocorticoid to mineralocorticoid receptors mRNA expression within the adrenals (C). mRNA quantification of the corticotrophin rele…

Rats that received sham surgery did not receive any treatment (n = 10). Rats in the endometriosis groups received antalarmin (n = 20) or vehicle treatment (n = 20) during one week after surgery until 53 days before behavioral testing. (A an…

(A) Total distance traveled during the first 5 minutes of the open field test and (B) time spent in the center of the arena. (C) Total distance traveled in the zero maze during the 5 min duration of the test and the amount of time spent in …

Characteristics of endometriosis vesicles at seven days after auto-transplantation surgery.

Rats in Experiment 1 received endometriosis or sham surgery and were allowed to progress for 7 days. Rats in Experiment 2 received sham surgery or endometriosis. Then the rats from the endometriosis group were injected with either vehicle (…

(A) The percent of implants that developed into vesicles was significantly lower in the antalarmin treated group (n = 20) compared to the vehicle control group (n = 20). (B) The total weight of all vesicles per rat was smaller for the antal…

(A) corticotropin releasing hormone (CRH), n = 14 vehicle and n = 9 antalarmin. (B) Urocortin 1 peptide, n = 9 in both groups. Data normalized to the uterus of sham rats. * represents p< 0.05 compared to sham rats’ uterus.

(A) corticotropin releasing hormone receptor type 1 (CRHR1). (B) corticotropin releasing hormone receptor type 2 (CRHR2). (C) Glucocorticoid receptor (GR). Data normalized to the uterus of sham rats. * represents p< 0.05 compared to sham ra…

(A) After seven days of treatment, antalarmin treatment significantly decreased the weight of the rats and this difference persisted for two additional days after the drug treatment has stopped. (B) During the subsequent weeks, the antalarm…

(A) At 7 days after the autotransplantation surgery to induce endometriosis, we observed a significant two-fold increase in CRHR1 mRNA within endometriosis vesicles only (n = 7 per group). (B) Western blot analysis confirmed the qRT-PCR obs…