qRT-PCR and Western Blot analysis of CRHR1 within endometriosis vesicles and uterus of the endometriosis rats and sham rats.

Annelyn Torres-Reverón (5650028), Leslie L. Rivera-Lopez (5963024), Idhaliz Flores (5650034), Caroline B. Appleyard (5650037)
2018 · doi:10.1371/journal.pone.0197698.g004 · W6923280390
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This study investigated the expression of corticotropin releasing hormone receptor 1 (CRHR1) in a rat model of endometriosis using autotransplantation surgery. Researchers compared CRHR1 levels in endometriosis vesicles and uterine tissue against sham-operated controls via qRT-PCR and Western blot analysis. The results demonstrated a significant two-fold increase in both mRNA and protein levels of CRHR1 specifically within the endometriosis vesicles seven days post-surgery, with no such elevation observed in the uterus or sham groups. This paper is centrally about endometriosis — specifically the upregulation of CRHR1 in ectopic lesions within an animal model.

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Abstract

(A) At 7 days after the autotransplantation surgery to induce endometriosis, we observed a significant two-fold increase in CRHR1 mRNA within endometriosis vesicles only (n = 7 per group). (B) Western blot analysis confirmed the qRT-PCR observation showing a significant increase in CRHR1 within the endometriosis vesicles only. (C) Sample Western blot bands with the respective marker. CRHR1 was observed just above the 50 kDa marker and β-actin above the 37kDa marker. * represents p< 0.05 compared to sham group. ** represents p< 0.01 on a post-hoc analysis compared to the two other groups. Sham group n = 7, endo uterus, n = 7, endo vesicles n = 9.
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Antagonizing the corticotropin releasing hormone receptor 1 with antalarmin reduces the progression of endometriosis Fig 4 qRT-PCR and Western Blot analysis of CRHR1 within endometriosis vesicles and uterus of the endometriosis rats and sham rats. (A) At 7 days after the autotransplantation surgery to induce endometriosis, we observed a significant two-fold increase in CRHR1 mRNA within endometriosis vesicles only (n = 7 per group). (B) Western blot analysis confirmed the qRT-PCR observation showing a significant increase in CRHR1 within the endometriosis vesicles only. (C) Sample Western blot bands with the respective marker. CRHR1 was observed just above the 50 kDa marker and β-actin above the 37kDa marker. * represents p< 0.05 compared to sham group. ** represents p< 0.01 on a post-hoc analysis compared to the two other groups. Sham group n = 7, endo uterus, n = 7, endo vesicles n = 9.

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endometriosis

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