qRT-PCR and Western Blot analysis of CRHR1 within endometriosis vesicles and uterus of the endometriosis rats and sham rats.

(A) At 7 days after the autotransplantation surgery to induce endometriosis, we observed a significant two-fold increase in CRHR1 mRNA within endometriosis vesicles only (n = 7 per group). (B) Western blot analysis confirmed the qRT-PCR observation showing a significant increase in CRHR1 within the endometriosis vesicles only. (C) Sample Western blot bands with the respective marker. CRHR1 was observed just above the 50 kDa marker and β-actin above the 37kDa marker. * represents p< 0.05 compared to sham group. ** represents p< 0.01 on a post-hoc analysis compared to the two other groups. Sham group n = 7, endo uterus, n = 7, endo vesicles n = 9.