Soluble interleukin-1 receptor type II blocks monocyte chemotactic protein-1 secretion by U937 cells in response to peripheral blood serum of women with endometriosis

Abdelaziz Kharfi, Ali Akoum
Fertility and Sterility · 2002 · doi:10.1016/s0015-0282(02)03335-6 · PMID:12372465
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Abstract

OBJECTIVE: To assess the ability of peripheral blood serum from women with endometriosis to induce monocyte chemotactic protein-1 (MCP-1) secretion by monocytes and the putative role of the interleukin-1 (IL-1) system in endometriosis-associated monocyte activation. DESIGN: Cultures of U937 monocytic cells exposed to serum from normal women (control group) or women with endometriosis. SETTING: Gynecology clinic and human reproduction research laboratory. PATIENT(S): Seventy-nine women with endometriosis and 38 control women with no evidence of endometriosis at laparoscopy. INTERVENTION(S): Peripheral blood obtained a few days before laparoscopy. MAIN OUTCOME MEASURE(S): MCP-1 secretion in the culture medium and serum concentrations of soluble IL-1 receptor type II (sIL-1RII), IL-1beta, and IL-1alpha by ELISA or by enzyme immunometric assay. RESULT(S): Serum concentrations of sIL-1RII were significantly lower in women with stage I-II endometriosis than in control women, whereas serum concentrations of IL-1beta and IL-1alpha were comparable between the two groups. The serum of women with endometriosis induced higher secretion of MCP-1 by U937 cells than that of control women, particularly in the initial stages of endometriosis (stages I-II), and recombinant IL-1RII (rIL-1RII) significantly blocked that secretion. CONCLUSION(S): These findings point toward a deficiency in the mechanisms involved in the down-regulation of IL-1 actions at the systemic level and reveal sIL-1RII as a key factor involved in that process.

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Condition tags

mesh:D004715endometriosis

MeSH descriptors

Chemokine CCL2 Endometriosis Monocytes Receptors, Interleukin-1 Chemokine CCL2 Culture Media Endometriosis Enzyme-Linked Immunosorbent Assay Female Humans Immunoenzyme Techniques Monocytes Receptors, Interleukin-1 Receptors, Interleukin-1 Receptors, Interleukin-1 Receptors, Interleukin-1 Type II Recombinant Proteins Recombinant Proteins Solubility U937 Cells

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