Estrogen biosynthesis in human uterine adenomyosis
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Aromatase activity in human adenomyosis tissue was higher than in normal myometrium or endometrium but lower than in tumor tissue, and was inhibited by danazol but stimulated by progesterone.
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Abstract
Estrogen biosynthesis (aromatase activity) was investigated in human adenomyosis tissue and compared with that of the normal myometrium, endometrium, and endometrial cancer tissues. Homogenates were incubated with [1,2,6,7-3H]androstenedione and NADPH at 37 degrees C for 1 h. After stopping the enzymatic reaction with ethyl acetate, [4-14C]estrone and [4-14C]estradiol-17 beta were added to the incubated sample. Estrone and estradiol were purified and identified by Bio-Rad AG1-X2 column chromatography, thin-layer chromatography and co-crystallization. Estrogen formed in the incubated sample was calculated from the 3H/14C ratio of the final crystal. The value for estrone formed from androstenedione was 52-132 fmol.h-1.g-1 wet weight. Aromatase activity in the adenomyosis tissues was higher than that in normal endometrial or myometrial tissues, but lower than that found in myometrial or endometrial tumour tissue. Furthermore, we investigated the effect of danazol, progesterone, and medroxyprogesterone acetate on adenomyosis cells in primary cultures. Aromatase activity in adenomyosis was blocked by danazol, but stimulated by progesterone and MPA. These results indicate that aromatase activity in adenomyosis may contribute to the growth of the ectopic endometrial tissue which occurs in this disease.
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Cited by (39)
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- Contemporary evaluation of women and girls with abnormal uterine bleeding: FIGO Systems 1 and 2 2023
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- Functional Implications of Estrogen and Progesterone Receptors Expression in Adenomyosis, Potential Targets for Endocrinological Therapy 2022
- Adenomyosis; Diagnosis, Clinical Findings and Current Approaches to Treatment 2022
- Pituitary suppression with GnRH agonists before ART may be insufficient to treat women with severe adenomyosis 2022
- Molecular Targets for Nonhormonal Treatment Based on a Multistep Process of Adenomyosis Development 2022
- Physiopathologie de l’adénomyose : une énigme non résolue 2021
- Celecoxib, a selective COX‑2 inhibitor, markedly reduced the severity of tamoxifen‑induced adenomyosis in a murine model 2020
- Immunological changes associated with adenomyosis: a systematic review 2020
- Transmembrane G protein-coupled receptor 30 gene polymorphisms and uterine adenomyosis in Korean women 2019
- Coexistence of adenomyosis, adenocarcinoma, endometrial and myometrial lesions in resected uterine specimens 2018
- Pathogenesis of adenomyosis: an update on molecular mechanisms 2017
- Epithelial-to-mesenchymal transition in the development of adenomyosis 2015
- The Pathophysiology of Adenomyosis 2015
- Adenomyosis: What is New? 2015
- Ultrasound studies of the endometrial-myometrial junction for the diagnosis of adenomyosis and endometrial cancer 2014
- Exposure to ethinyl estradiol prenatally and/or after sexual maturity induces endometriotic and precancerous lesions in uteri and ovaries of mice 2012
- The pathophysiology of uterine adenomyosis: an update 2012
- Expression of Cyclooxygenase-2 and Matrix Metalloproteinase-2 in Adenomyosis and Endometrial Polyps and its Correlation With Angiogenesis 2009
- Hormonal treatments for adenomyosis 2007
- Expression and regulation of estrogen-converting enzymes in ectopic human endometrial tissue 2007
- Adenomyosis: the pathophysiology of an oestrogen-dependent disease 2006
- Transvaginal ultrasound for diagnosis of adenomyosis: a review 2006
- Effect of the menstrual cycle and oral contraceptives on aromatase and cyclooxygenase-2 expression in adenomyosis 2006
- Treatment of Endometrial Hyperplasia with a Danazol-Releasing Intrauterine Device: A Prospective Study 2004
- Gonadotropin-releasing hormone agonist and danazol normalize aromatase cytochrome P450 expression in eutopic endometrium from women with endometriosis, adenomyosis, or leiomyomas 2003
- Adenomyosis 2003
- Endometriosis: the pathophysiology as an estrogen-dependent disease 2002
- Prevalence and risk factors of adenomyosis at hysterectomy 2001
- Detection of aromatase cytochrome P-450 in endometrial biopsy specimens as a diagnostic test for endometriosis 1999
- Expression of Aromatase Cytochrome P450 in Eutopic Endometrium and Its Application as a Diagnostic Test for Endometriosis 1999
- Pathophysiology of adenomyosis 1998
- An effective minimally invasive method of treating adenomyosis by interstitial laser photocoagulation with the KTP laser 1997
- Expression of Aromatase Cytochrome P450 Protein and Messenger Ribonucleic Acid in Human Endometriotic and Adenomyotic Tissues but not in Normal Endometrium1 1997
- Peri- and Postmenopausal Abnormal Bleeding 1994
- Evidence for estrogen synthesis in adenomyotic tissues 1993
- Inhibitory effect of a new androstenedione derivative, 14α-hydroxy-4-androstene-3,6,17-trione (14α-OHAT) on aromatase activity of human uterine tumors 1990
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