Quantitative enzyme immunoassay and semiquantitative immunohistochemistry of oestrogen and progesterone receptors in endometriotic tissue and endometrium

Bergqvist A, A Bergqvist, Agneta Bergqvist, M Fernö, Fernö M, Skoog L, Lambert Skoog, L Skoog
Journal of clinical pathology · 1997 · vol. 50(6) , pp. 496–500 · doi:10.1136/jcp.50.6.496 · PMID:9378817 · PMC499985 · W2054036831
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This study compared quantitative enzyme immunoassay and semiquantitative immunocytochemistry for measuring estrogen and progesterone receptors in endometriotic tissue and endometrium.

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Abstract

BACKGROUND: The capacity of different tissues to respond to steroids has been evaluated mainly according to the concentrations of oestrogen (ER) and progesterone (PR) receptors, which may be assayed either in tissue homogenates or histochemically. These two principles have been used in different studies of endometriotic tissue, giving somewhat different results, probably because the tissue is heterogeneously mixed with fibrotic tissue and blood cells to various degrees. AIMS: To compare a quantitative and a qualitative, semiquantitative immunocytochemical assay for ER and PR in a heterogenous tissue (endometriotic) and a similar but more homogenous tissue (endometrium). METHODS: ER and PR concentrations were measured using enzyme immunoassay (EIA) and immunocytochemistry (ICA) in the same tissue samples (endometriotic tissue from 31 women and endometrium from 19 of the same women). RESULTS: There was a significant correlation between PR concentration (using EIA) and semiquantitation of PR using PRICA in endometrial epithelium (p = 0.028). The correlation between the two techniques for ER concentration was not significant. In endometriotic tissue no correlation was found between the two techniques for ER or PR. CONCLUSIONS: These data show that in heterogeneous tissues like endometriotic tissue different techniques for measuring steroid receptors may produce substantially different results. The two techniques (EIA and ICA) are complementary and a combination of the two techniques might aid in determining optimal treatment.

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Condition tags

endometriosis

MeSH descriptors

Endometriosis Endometriosis Endometrium Receptors, Estrogen Receptors, Progesterone Adult Adult Endometrium Endometrium Female Female Humans Humans Immunoenzyme Techniques Immunoenzyme Techniques Immunohistochemistry Immunohistochemistry Immunohistochemistry Receptors, Estrogen Receptors, Estrogen

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Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. Outer rings show 2-hop neighbours — papers reached through the immediate citers/citees. [ collapse to 1-hop ]

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