Localization of transforming growth factor beta isoforms TGF-beta 1, TGF-beta 2, and TGF-beta 3 in surgically induced endometriosis in the rat
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Endometriotic implants in rats demonstrated TGF-beta 1 and TGF-beta 3 immunoreactivity in most cell types except stromal cells, with inflammatory cells showing the highest intensity.
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Abstract
OBJECTIVE: To elucidate the presence and cellular distribution of transforming growth factor beta (TGF-beta) in surgically induced endometriosis in the rat.
METHODS: Endometriosis was induced by implanting pieces of uterine fragments in the mesenteric region adjacent to a blood vessel for a period of 4-6 weeks. The endometrial implants were removed and processed for immunohistochemical localization using isoform-specific polyclonal antibodies to TGF-beta 1, TGF-beta 2, and TGF-beta 3.
RESULTS: All the cell types in the endometrial implants with the exception of stromal cells immunostained for TGF-beta 1 and TGF-beta 3, but not TGF-beta 2. The inflammatory cells that were infiltrated among endometriotic stromal cells and implant-associated cysts contained the highest immunostaining intensity for TGF-beta s 1-3, followed by luminal and glandular epithelial cells, fibroblasts of the fibrous adhesions, and endothelial and smooth-muscle cells of arterioles. The immunostaining intensity of TGF-beta 1 was substantially higher than that of TGF-beta 3 and TGF-beta 2, and intensity was similar to the endometrial tissue from cycling rats in diestrus II for TGF-beta 1 and estrus for TGF-beta 2 and TGF-beta 3. In the cycling rats, the order of immunostaining intensity in the endometrium was TGF-beta 1, TGF-beta 3, and TGF-beta 2, respectively, with a higher intensity in diestrus II and proestrus than in diestrus I and estrus.
CONCLUSION: The results indicate that endometrial implants in surgically induced endometriosis contain immunoreactive TGF-beta s, which may imply a possible paracrine/autocrine role for the action of TGF-beta in the maintenance of viable endometriotic tissue and the development of fibrous adhesions associated with the implants.
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Cited by (21)
- A Preliminary Investigation of the Roles of Endometrial Cells in Endometriosis Development via In Vitro and In Vivo Analyses 2024
- Lysine acetyltransferase 14 mediates TGF-β-induced fibrosis in ovarian endometrioma via co-operation with serum response factor 2024
- Increased Expression of TGF-β1 Contributes to the Downregulation of Progesterone Receptor Expression in the Eutopic Endometrium of Infertile Women with Minimal/Mild Endometriosis 2023
- MiR-143-3p facilitates motility and invasiveness of endometriotic stromal cells by targeting VASH1/TGF-β signaling 2022
- Targeted inhibition of TAK1 abrogates TGFβ1 non-canonical signaling axis, NFκB/Smad7 inhibiting human endometriotic cells proliferation and inducing cell death involving autophagy 2021
- Cancer-associated mutations in endometriosis: shedding light on the pathogenesis and pathophysiology 2019
- Inside the Endometrial Cell Signaling Subway: Mind the Gap(s) 2018
- The involvement of multifunctional TGF-β and related cytokines in pathogenesis of endometriosis 2018
- Hypoxia Promotes Ectopic Adhesion Ability of Endometrial Stromal Cells via TGF-β1/Smad Signaling in Endometriosis 2018
- The role of TGF-β in the pathophysiology of peritoneal endometriosis 2017
- Transforming growth factor beta1 from endometriomas promotes fibrosis in surrounding ovarian tissues via Smad2/3 signaling 2017
- Peritoneal VEGF-A expression is regulated by TGF-β1 through an ID1 pathway in women with endometriosis 2015
- Endometrial-peritoneal interactions during endometriotic lesion establishment 2008
- Basal release of urokinase plasminogen activator, plasminogen activator inhibitor-1, and soluble plasminogen activator receptor from separated and cultured endometriotic and endometrial stromal and epithelial cells 2005
- Behaviour of cytokine levels in serum and peritoneal fluid of women with endometriosis 2002
- Expression of the cyclin-dependent kinase inhibitor p27Kip1 in eutopic endometrium and peritoneal endometriosis 2001
- Cytokine profiles in autologous peritoneal fluid and peripheral blood of women with deep and superficial endometriosis 2001
- Macrophage secretory products and sperm zona pellucida binding 2001
- Adhesion prevention in the surgical treatment of pelvic endometriosis 2001
- Macrophage derived growth factors modulate Fas ligand expression in cultured endometrial stromal cells: a role in endometriosis 1999
- Increased mast cell density in peritoneal endometriosis compared with eutopic endometrium with endometriosis 1998
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