Effect of surface-enhanced laser desorption/ionization time-of-flight mass spectrometry on identifing biomarkers of endometriosis

Hong Zhang, Jie Feng, Xiaohong Chang, Xiao-hong Chang, Zhongxing Li, Zhong-xing Li, Xiaoyi Wu, Xiao-yi Wu, Heng Cui
Chinese medical journal · 2009 · vol. 122(4) , pp. 373–376 · doi:10.3760/cma.j.issn.0366-6999.2009.04.003 · PMID:19302738 · W2408940320
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Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry identified 24 differentially expressed serum proteins in endometriosis patients, achieving high sensitivity and specificity in diagnosing the condition.

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Abstract

BACKGROUND: Endometriosis is a common gynecological disease. This study aimed to screen proteins that were expressed differently in patients with endometriosis versus normal controls using proteomic techniques, surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS). METHODS: Protein chip SELDI-TOF-MS combines the advantages of microarray and mass spectrometry, and can screen latent markers in sera of patients with endometriosis. Serum samples from patients and normal volunteers were analyzed by SELDI-TOF-MS. RESULTS: After comparing the serum protein spectra of 36 patients with 24 normal controls, 24 differently expressed potential biomarkers (P < 0.01) were identified. Using Biomarker Pattern software, we established a tree model of the 60 serum protein spectra. When using the three biomarkers to classify the samples, the sensitivity for diagnosing endometriosis was 91.7%, specificity was 95.8%, and coincidence rate was 93.3%. Then we used serum samples from 12 patients and 8 normal controls to validate the tree model and report the sensitivity for diagnosing endometriosis was 91.7%, specificity was 75%, and coincidence rate was 85%. CONCLUSIONS: SELDI-TOF-MS may be a useful tool in high-risk population screening for endometriosis. The identification and application of the biomarkers need to further study.

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Condition tags

mesh:D004715endometriosis

MeSH descriptors

Biomarkers Endometriosis Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Biomarkers Endometriosis Endometriosis Female Humans Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

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