Macrophages are activated by 17b-estradiol: possible permission role in endometriosis

Hong M, Min Hong, Quan Zhu, Zhu Q, Zhu Quan
Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie · 2004 · vol. 55(5) , pp. 385–391 · doi:10.1078/0940-2993-00335 · PMID:15088640 · W2051058490
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This study investigated the activation of macrophages by 17b-estradiol and its potential role in endometriosis.

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Abstract

On the basis of the common occurrence of high concentration of estrogen and activated macrophages in patients with endometriosis, we postulate that interaction between 17beta-estradiol and macrophage may be an important affair in endometriosis. So our study was focused on the effect of 17beta-estradiol on macrophage. First morphology of macrophages was examined with environmental scanning electron microscopy. Increased size, extension of more microvilli, expression of retraction fibers and elaboration of membrane ruffles were detected in 17beta-estradiol treated macrophages. Then Nitrate and nitrite level in the supernatant was measured by the method of Griess and iNOS expression was analyzed using immunohistochemical staining. It showed that 17beta-estradiol could induce NO release from peritoneal macrophages and expression of iNOS was increased. Also more TNF-alpha in supernatant that was measured by MTT via L929 cell was produced by macrophages under the inducing of 17beta-estradiol. Furthermore, [Ca2+]i, which was viewed by microscope in a laser scanning confocal unit, elevated 39.8% in peritoneal macrophages after 17beta-estradiol 100 nmol/L treated. The results above demonstrated that peritoneal macrophage had been activated in both morphology and cytokine line when interaction with 17beta-estradiol, which indicated that macrophage activated by 17beta-estradiol might play a permission role in development of endometriosis.

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Condition tags

endometriosis

MeSH descriptors

Endometriosis Endometriosis Endometriosis Estradiol Macrophages, Peritoneal Animals Calcium Calcium Calcium Signaling Calcium Signaling Cells, Cultured Estradiol Female Immunohistochemistry Macrophages, Peritoneal Macrophages, Peritoneal Macrophages, Peritoneal Mice Mice, Inbred BALB C Microscopy, Electron, Scanning

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Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

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