Isolation and purification of human endometrial stromal and glandular cells using immunomagnetic microspheres

S. Fernández-Shaw, S C Shorter, C E Naish, Caroline Naish, D H Barlow, David H. Barlow, P M Starkey
Human Reproduction · 1992 · vol. 7(2) , pp. 156–161 · doi:10.1093/oxfordjournals.humrep.a137609 · PMID:1349612 · W2135499528
Lab / animal OA: closed CC0 ⤵ 14 in-corpus citations
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Immunomagnetic microspheres coated with anti-Thy-1 antibody successfully isolated 98% pure stromal cells and 82% pure glandular cells from human endometrium, both of which proliferated in vitro.

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Abstract

Isolation of pure preparations of the different cell populations of human endometrium is a prerequisite for studies of in-vitro function. Sieving of dispersed endometrial cells, followed by adsorption onto immunomagnetic microspheres coated with antibody to Thy-1 was used to separate glandular and stromal cells. The purity of these cell populations was checked with antibodies to cytokeratin and Thy-1. The stromal cells were 98% pure and 90% viable, gland cells were 82% pure with 76% viability. The purified cells were able to proliferate in vitro as shown by thymidine incorporation.

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MeSH descriptors

Cell Separation Antigens, Surface Antigens, Surface Cells, Cultured Cell Separation Centrifugation Endometrium Endometrium Endometrium Epithelial Cells Female Humans Immunohistochemistry Magnetics Membrane Glycoproteins Membrane Glycoproteins Microspheres Thy-1 Antigens Thymidine Thymidine

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