Signal transduction pathways involved in macrophage migration induced by peritoneal fluid chemotactic factors in stages I and II endometriosis

Maria Claudia Perez, Perez MC, María Claudia Pérez, Bodine PV, Peter V N Bodine, Leiva MC, Maria C Leiva, María C. Leiva, Keith Isaacson, Keith B Isaacson, Isaacson KB, Barry S. Komm, Komm BS
Fertility and sterility · 2002 · vol. 77(6) , pp. 1261–1268 · doi:10.1016/s0015-0282(02)03130-8 · PMID:12057738 · W2001954730
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This study investigated the signal transduction pathways activated by peritoneal fluid chemotactic factors, which mediate macrophage migration in patients with early-stage endometriosis.

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Abstract

OBJECTIVE: To explore the role of G-protein coupled signaling pathways in activation of macrophage migration in endometriosis stages I and II. DESIGN: Case controlled study. SETTING: University hospital. PATIENT(S): Fifteen patients undergoing laparoscopy for elective sterilization (n = 5) or for diagnosis of endometriosis stages I and II associated with infertility (n = 10). INTERVENTION(S): Peritoneal fluid samples were collected during laparoscopy. MAIN OUTCOME MEASURE(S): Macrophage migration induced by peritoneal fluid from patients with endometriosis stages I and II (PF SI-II) and potential G-protein coupled receptors and second messengers involved in macrophage activation. RESULT(S): Potential G-protein coupled receptors and second messengers involved in macrophage activation were evaluated after incubation of U-937 cells differentiated into macrophages with inhibitors of phospholipase A and C, adenylate cyclase, and protein kinase A and C. Macrophage chemotactic activity induced by PF SI-II was inhibited in the presence of a phospholipase C and A2 inhibitor (IC50= 30 microM) and after treatment with myristoylated protein kinase C peptide inhibitor (50 nM). An increase in inositol phosphate (IP3) was also observed in macrophages exposed to PF SI-II. Activation of multiple G-proteins in macrophages was examined after exposure of cells to PF SI-II in the presence and absence of Bordettela pertussis and cholera toxins. No effect on macrophage migration was observed. CONCLUSION(S): Macrophage chemotaxis induced by PF SI-II appears to involve activation of pertussis toxin-insensitive G-protein coupled receptors in macrophages. Our data suggest that these events lead to subsequent activation of phospholipases followed by generation of IP3 and potential mobilization of intracellular Ca2+. Subsequent phosphorylation of target proteins by protein kinase C may regulate the chemotactic responses. The adenylate cyclase pathway does not appear to play a role in this process.

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Condition tags

endometriosis

MeSH descriptors

Ascitic Fluid Chemotactic Factors Endometriosis Macrophages Signal Transduction Signal Transduction Ascitic Fluid Ascitic Fluid Case-Control Studies Case-Control Studies Cell Line Cell Line Cell Movement Cell Movement Cell Movement Chemotactic Factors Chemotactic Factors Endometriosis Endometriosis Endometriosis

Citation neighborhood (2-hop)

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. Outer rings show 2-hop neighbours — papers reached through the immediate citers/citees. [ collapse to 1-hop ]

References (37)

Cited by (3)

SciLite annotations

chemicals 3
peptide inositol phosphate hexose + c4h5n3o2
organisms 2
strain 18323 tholera

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