Decreased expression of Beclin 1 in eutopic endometrium of women with adenomyosis

Yuefang Ren, Ren Y, Mu L, Lin Mu, Ding X, Xiaoyan Ding, Zheng W, Wei Zheng
Archives of gynecology and obstetrics · 2009 · vol. 282(4) , pp. 401–406 · doi:10.1007/s00404-009-1280-0 · PMID:19921231 · W2010130149
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Beclin 1 mRNA and protein expression were significantly lower in the eutopic endometria of women with adenomyosis and negatively correlated with serum CA125 and pelvic pain.

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This study examined whether the autophagy-related gene Beclin 1 shows altered expression in eutopic endometrium of women with adenomyosis and how expression relates to clinical features. Tissue was collected from 30 women with adenomyosis and 32 healthy controls undergoing surgery for benign indications, and Beclin 1 mRNA and protein expression were measured in cultured endometrial stromal cells and in the endometrial tissues using RT-PCR and western blotting. Beclin 1 mRNA and protein levels were significantly lower in adenomyosis samples than in controls, and within the adenomyosis group Beclin 1 protein expression in eutopic endometrial tissues was negatively correlated with serum CA125 and pelvic pain. The paper relates to endometriosis because it discusses Beclin 1 autophagy involvement in endometriosis pathogenesis and progression, and it is centrally about adenomyosis—showing decreased Beclin 1 in eutopic endometrium of women with adenomyosis.

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Abstract

PurposeResearchers have launched a new area of febrile investigations on the autophagy-related gene Beclin 1. Our aim is to investigate whether Beclin 1 expression is altered in eutopic endometrium of women with adenomyosis and its association with clinical characteristics.MethodsWe collected tissue samples from the eutopic endometria of 30 women with adenomyosis and 32 healthy women undergoing surgery for benign indications. We cultured the stromal cells of the eutopic endometria. Beclin 1 expression of the cultured stromal cells and tissues was assessed by reverse transcription polymerase chain reaction and western blot analysis.ResultsBeclin 1 messenger RNA (mRNA) expression in cultured stromal cells of eutopic endometria and endometrial tissues of women with adenomyosis was significantly lower than that of controls (P < 0.05). Beclin 1 protein expression in cultured stromal cells of eutopic endometria and endometrial tissues of adenomyosis was also significantly lower compared with that of controls (P < 0.01). Beclin 1 protein expression in eutopic endometrial tissues was negatively correlated with serum CA125 (r = -0.307, P = 0.015), and pelvic pain (r = -0.542, P = 0.000).ConclusionsThe study revealed Beclin 1 mRNA and protein expression were significantly decreased in eutopic endometria of women with adenomyosis. Moreover, Beclin 1 was negatively correlated with serum CA125 and pelvic pain. Beclin 1 might contribute to the pathogenesis and progression of endometriosis. Further research on autophagy of adenomyosis is required.
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Abstract

Purpose Researchers have launched a new area of febrile investigations on the autophagy-related gene Beclin 1. Our aim is to investigate whether Beclin 1 expression is altered in eutopic endometrium of women with adenomyosis and its association with clinical characteristics.

Methods

We collected tissue samples from the eutopic endometria of 30 women with adenomyosis and 32 healthy women undergoing surgery for benign indications. We cultured the stromal cells of the eutopic endometria. Beclin 1 expression of the cultured stromal cells and tissues was assessed by reverse transcription polymerase chain reaction and western blot analysis.

Results

Beclin 1 messenger RNA (mRNA) expression in cultured stromal cells of eutopic endometria and endometrial tissues of women with adenomyosis was significantly lower than that of controls (P < 0.05). Beclin 1 protein expression in cultured stromal cells of eutopic endometria and endometrial tissues of adenomyosis was also significantly lower compared with that of controls (P < 0.01). Beclin 1 protein expression in eutopic endometrial tissues was negatively correlated with serum CA125 (r = −0.307, P = 0.015), and pelvic pain (r = −0.542, P = 0.000).

Conclusions

The study revealed Beclin 1 mRNA and protein expression were significantly decreased in eutopic endometria of women with adenomyosis. Moreover, Beclin 1 was negatively correlated with serum CA125 and pelvic pain. Beclin 1 might contribute to the pathogenesis and progression of endometriosis. Further research on autophagy of adenomyosis is required. Similar content being viewed by others

References

Parazzini F, Vercellini P, Panazza S, Chatenoud L, Oldani S, Crosignani PG (1997) Risk factors for adenomyosis. Hum Reprod 12:1275–1279 Devlieger R, D’Hooghe T, Timmerman D (2003) Uterine adenomyosis in the infertility clinic. Hum Reprod Update 9:139–147 Edinger AL, Thompson CB (2004) Death by design: apoptosis, necrosis and autophagy. Curr Opin Cell Biol 16:663–669 Tsujimoto Y, Shimizu S (2005) Another way to die: autophagic programmed cell death. Cell Death Differ 12(Suppl 2):1528–1534 Aita VM, Liang XH, Murty VV, Pincus DL, Yu W, Cayanis E et al (1999) Cloning and genomic structure of beclin 1, a candidate tumor suppressor gene on chromosome 17q21. Genomics 59:59–65 Miracco C, Cosci E, Oliveri G, Luzi P, Pacenti L, Monciatti I et al (2007) Protein and mRNA expression of autophagy gene Beclin 1 in human brain tumours. Int J Oncol 30:429–436 Wang ZH, Xu L, Duan ZL, Zeng LQ, Yan NH, Peng ZL (2007) Beclin 1-mediated macroautophagy involves regulation of caspase-9 expression in cervical cancer HeLa cells. Gynecol Oncol 107:107–113 Liang XH, Jackson S, Seaman M, Brown K, Kempkes B, Hibshoosh H et al (1999) Induction of autophagy and inhibition of tumorigenesis by beclin 1. Nature 402:672–676 Tsai SJ, Wu MH, Lin CC, Sun HS, Chen SM (2001) Regulation of steroidogenic acute regulatory protein expression and progesterone production in endometriotic stromal cells. J Clin Endocrinol Metab 86:5765–5773 Wing LY, Chuang PC, Wu MH, Chen HM, Tsai SJ (2003) Expression and mitogenic effect of fibroblast growth factor-9 in human endometriotic implant is regulated by aberrant production of estrogen. J Clin Endocrinol Metab 88:5547–5554 Qu X, Yu J, Bhagat G, Furuya N, Hibshoosh H, Troxel A et al (2003) Promotion of tumorigenesis by heterozygous disruption of the beclin 1 autophagy gene. J Clin Invest 112:1809–1820 Mathew R, Kongara S, Beaudoin B, Karp CM, Bray K, Degenhardt K et al (2007) Autophagy suppresses tumor progression by limiting chromosomal instability. Genes Dev 21:1367–1381 Ota H, Igarashi S, Hatazawa J, Tanaka T (1998) Is adenomyosis an immune disease? Hum Reprod Update 4:360–367 Wang F, Wen Z, Li H, Yang Z, Zhao X, Yao X (2008) Human leukocyte antigen-G is expressed by the eutopic and ectopic endometrium of adenomyosis. Fertil Steril 90:1599–1604 Paludan C, Schmid D, Landthaler M, Vockerodt M, Kube D, Tuschl T et al (2005) Endogenous MHC class II processing of a viral nuclear antigen after autophagy. Science 307:593–596 Furuya N, Yu J, Byfield M, Pattingre S, Levine B (2005) The evolutionarily conserved domain of Beclin 1 is required for Vps34 binding, autophagy and tumor suppressor function. Autophagy 1:46–52 Liang C, Feng P, Ku B, Dotan I, Canaani D, Oh BH et al (2006) Autophagic and tumour suppressor activity of a novel Beclin1-binding protein UVRAG. Nat Cell Biol 8:688–699 Oberstein A, Jeffrey PD, Shi Y (2007) Crystal structure of the Bcl-XLBeclin 1 peptide complex: Beclin 1 is a novel BH3-only protein. J Biol Chem 282:13123–13132 Sun Y, Zhang J, Peng ZL (2009) Beclin1 induces autophagy and its potential contributions to sensitizes SiHa cells to carboplatin therapy. Int J Gynecol Cancer 19:772–776 Qu X, Zou Z, Sun Q, Luby-Phelps K, Cheng P, Hogan RN et al (2007) Autophagy gene-dependent clearance of apoptotic cells during embryonic development. Cell 128:931–946 Akar U, Chaves-Reyez A, Barria M, Tari A, Sanguino A, Kondo Y et al (2008) Silencing of Bcl-2 expression by small interfering RNA induces autophagic cell death in MCF-7 breast cancer cells. Autophagy 4:669–679 Kim DK, Yang JS, Maiti K, Hwang JI, Kim K, Seen D et al (2009) A gonadotropin-releasing hormone-II antagonist induces autophagy of prostate cancer cells. Cancer Res 69:923–931 Gründker C, Günthert AR, Millar RP, Emons G (2002) Expression of gonadotropin-releasing hormone II (GnRH-II) receptor in human endometrial and ovarian cancer cells and effects of GnRH-II on tumor cell proliferation. J Clin Endocrinol Metab 87:1427–1430 John S, Nayvelt I, Hsu HC, Yang P, Liu W, Das GM et al (2008) Regulation of estrogenic effects by beclin 1 in breast cancer cells. Cancer Res 68:7855–7863 Conflict of interest statement None. Author information Authors and Affiliations Corresponding author Rights and permissions About this article Cite this article Ren, Y., Mu, L., Ding, X. et al. Decreased expression of Beclin 1 in eutopic endometrium of women with adenomyosis. Arch Gynecol Obstet 282, 401–406 (2010). https://doi.org/10.1007/s00404-009-1280-0 Received: Accepted: Published: Issue date: DOI: https://doi.org/10.1007/s00404-009-1280-0

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Condition tags

endometriosischronic_pelvic_painadenomyosis

MeSH descriptors

Apoptosis Regulatory Proteins Endometriosis Endometrium Membrane Proteins RNA, Messenger Stromal Cells Adult Apoptosis Regulatory Proteins Autophagy Beclin-1 Blotting, Western CA-125 Antigen CA-125 Antigen CA-125 Antigen Case-Control Studies Endometriosis Endometriosis Endometrium Endometrium Female

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