The role of laser capture microdissection in the molecular-genetic investigation of endometriosis (Literature review and own experience)

L.V. Adamyan, L.G. Pivazyan, K.S. Kurbatova, R. Yu. Saryglar, A.Yu. Lupatov, A.V. Asaturova, M.V. Kuznetsova, D.Yu. Trofimov, A.A. Stepanian
In: Russian Journal of Human Reproduction · 2026 · vol. 32(3) , pp. 15 · doi:10.17116/repro20263203115 · W7167243102
article OA: closed CC0
Full text JSON View on OpenAlex View at publisher
⚙ AI-generated summary by claude@2026-07, 2026-07-07 ⓘ

This review analyzes laser capture microdissection's utility in isolating specific cell populations from heterogeneous endometriosis tissues for molecular-genetic investigation, detailing methods, applications, and future prospects.

One-sentence paraphrase of the abstract; not a substitute for reading it. No clinical advice. How this works

⚙ AI-generated deep summary by claude@2026-07, 2026-07-14 · read from full text ⓘ

This paper is a literature review with authors’ experience on how laser capture microdissection is used for molecular-genetic investigations in endometriosis, with attention to collecting specific tissue compartments for downstream analyses such as gene-expression profiling, and to methodological validation. It synthesizes prior studies that applied laser capture microdissection to eutopic versus ectopic endometrium and highlights technical considerations raised in the literature and the authors’ own practical work, while emphasizing that sample handling and protocol performance are major determinants of results. A key caveat explicitly noted across such work is that differences in method and tissue quality can affect molecular readouts from limited, precious specimens. Relevance to endometriosis: the entire review is centered on endometriosis and specifically discusses the use of laser capture microdissection in endometriosis molecular-genetic studies, including gene-expression profiling of endometriotic tissue.

Read from the paper's body, not the abstract. Not a substitute for reading the paper. No clinical advice. How this works

Abstract

Endometriosis is a gynecological disease affecting more than 190 million women of reproductive age worldwide, characterized by a complex molecular pathophysiology encompassing genetic, epigenetic, inflammatory, and immunological components. A fundamental methodological challenge in the study of endometriosis is the cellular heterogeneity of ectopic lesions: conventional analysis of bulk tissue specimens reflects averaged molecular profiles of mixed cell populations comprising glandular epithelium, stroma, fibrous and muscular tissue, and immune microenvironmental cells, substantially impeding the identification of cell-specific molecular alterations. This article presents an analysis of the capabilities of laser capture microdissection as a technology for the selective isolation of cell populations from heterogeneous tissues, enabling researchers to overcome this limitation. The methodological principles of the technology are described in detail, including critical parameters of biological material preparation, integration with downstream molecular analysis platforms, and the specificities of protocol standardization and validation across different tissue types. The broad applicability of laser capture microdissection in contemporary biomedical research is reviewed. Particular emphasis is placed on its application in endometriosis research specifically: data on transcriptomic profiling and differential gene expression analysis in the epithelial and stromal components of ectopic lesions are described; studies employing next-generation sequencing aimed at investigating somatic mutations, clonality, and mutational profiles of endometriotic cell populations are reviewed; and work in the field of multi-omics profiling across different disease forms is examined. The review also addresses existing methodological limitations of the technology and prospects for its further development, including integration with spatial transcriptomics, single-cell sequencing, and artificial intelligence-based approaches. Laser capture microdissection constitutes an instrument that opens fundamentally new avenues for investigating the molecular heterogeneity of endometriosis and establishing the foundation for the development of targeted, pathogenetically justified approaches to its diagnosis and treatment. This article also presents the authors’ own experience with the application of laser capture microdissection in endometriosis research. At the time of manuscript preparation, laser capture microdissection had been performed on 15 endometriotic lesion specimens obtained from patients with various forms of endometriosis, with separate isolation of epithelial and stromal components intended for subsequent molecular-genetic analysis.
Full text 15,584 characters · extracted from oa-doi-fallback · click to expand
Сайт издательства «Медиа Сфера» содержит материалы, предназначенные исключительно для работников здравоохранения. Закрывая это сообщение, Вы подтверждаете, что являетесь дипломированным медицинским работником или студентом медицинского образовательного учреждения. Результаты поиска: 0 Роль лазерной микродиссекции в молекулярно-генетическом исследовании эндометриоза (обзор литературы и собственный опыт) Журнал: Проблемы репродукции. 2026;32(3): 15‑31 Прочитано: 118 раз Как цитировать: Литература / References: - Эндометриоз. Клинические рекомендации. 2024. Одобрено Научно-практическим Советом Министерства здравоохранения Российской Федерации. Ссылка активна на 06.04.26. https://cr.minzdrav.gov.ru/view-cr/259_2 - Эндометриоз. Информационный бюллетень ВОЗ. 2024. Всемирная организация здравоохранения. Ссылка активна на 06.04.26. https://www.who.int/ru/news-room/fact-sheets/detail/endometriosis - Адамян Л.В., Алясова А.В., Пивазян Л.Г., Степанян А.А. Иммунологические аспекты эндометриоза: патофизиологические механизмы, диагностика, аутоиммунитет, таргетная терапия и модуляция. Проблемы репродукции. 2024;30(2):15-31. https://doi.org/10.17116/repro20243002115 - Адамян Л.В., Пивазян Л.Г., Маилова К.С. Роль ферроптоза в патогенезе и прогрессировании эндометриоза. История вопроса и новые данные. Проблемы репродукции. 2023;29(5):92-101. https://doi.org/10.17116/repro20232905192 - Adamyan L, Pivazyan L, Krylova E, Tarlakyan V, Murvatova K. Iron metabolism markers in peritoneal fluid of patients with endometri osis: systematic review and meta-analysis. Journal of Endometriosis and Uterine Disorders. 2024;5:100061. https://doi.org/10.1016/j.jeud.2024.100061 - Адамян Л.В., Пивазян Л.Г., Курбатова К.С., Маилова К.С., Степанян А.А. Оксидативный стресс, ферроптоз, соматические мутации, антиоксидантная терапия и эндометриоз: новый взгляд на проблему. Проблемы репродукции. 2024;30(6):32-44. https://doi.org/10.17116/repro20243006132 - Адамян Л.В., Малышев И.Ю., Пивазян Л.Г., Юрканова М.Д., Маилова К.С., Степанян А.А. Роль поляризации макрофагов в развитии эндометриоза и терапевтический потенциал ее модуляции. Проблемы репродукции. 2025;31(3):20-33. https://doi.org/10.17116/repro20253103120 - Adamyan L, Pivazyan L, Murvatova K, Tarlakyan V, Zarova E, Stepanian A, Mailova K. Association between the level of TGF- β expression and endometriosis: A systematic review and meta-analysis. Journal of Endometriosis and Uterine Disorders. 2025;9:100100. https://doi.org/10.1016/j.jeud.2024.100100 - Адамян Л.В., Кузнецова М.В., Пивазян Л.Г., Давыдова Ю.Д., Трофимов Д.Ю. Генетические аспекты эндометриоза и аденомиоза: современный взгляд на проблему. Проблемы репродукции. 2023;29(4-2):14-22. https://doi.org/10.17116/repro20232904214 - Becker CM, Bokor A, Heikinheimo O, Horne A, Jansen F, Kiesel L, King K, Kvaskoff M, Nap A, Petersen K, Saridogan E, Tomas setti C, van Hanegem N, Vulliemoz N, Vermeulen N; ESHRE En dometriosis Guideline Group. ESHRE guideline: endometriosis. Human Reproduction Open. 2022;2022(2):hoac009. https://doi.org/10.1093/hropen/hoac009 - Адамян Л.В., Пивазян Л.Г., Маилова К.С., Пешкова Ю.О., Степанян А.А. Экспериментальное моделирование, профилактика и лечение эндометриоз-ассоциированного спаечного процесса на клеточной линии NIH/3T3-фибробластов с применением бовгиалуронидазы азоксимер (in vitro). Проблемы репродукции. 2024;30(6):61-72. https://doi.org/10.17116/repro20243006161 - Adamyan L, Pivazyan L, Krylova E, Kurbatova K, Tarlakyan V, Stepanian A. Hyaluronic acid in the prevention of adhesions after gynecological surgery: systematic review and meta-analysis. Journal of Endometriosis and Uterine Disorders. 2024;6:100070. https://doi.org/10.1016/j.jeud.2024.100070 - Koninckx PR, Ussia A, Adamyan L, Wattiez A, Gomel V, Mar tin DC. Pathogenesis of endometriosis: the genetic/epigenetic the ory. Fertility and Sterility. 2019;111(2):327-340. https://doi.org/10.1016/j.fertnstert.2018.10.013 - Kennedy S. The genetics of endometriosis. European Journal of Obstetrics and Gynecology and Reproductive Biology. 1999;82:129-133. https://doi.org/10.1016/S0301-2115(98)00213-9 - Адамян Л.В., Серегина В.Ю., Пивазян Л.Г., Кузнецова М.В., Трофимов Д.Ю., Маилова К.С., Степанян А.А. Полногеномный поиск ассоциаций (Genome-Wide Association Studies) при эндометриозе: генетические механизмы и коморбидность. Проблемы репродукции. 2025;31(6):35-45. https://doi.org/10.17116/repro20253106135 - Adamyan L, Pivazyan L, Yurkanova M, Zarova E, Kuznetsova M, Mailova K, Trofimov D, Stepanian A. The role of somatic mutations in endometriosis: pathogenesis, progression, and fibrogenesis (narrative review). Fertility and Sterility Reviews. 2025;6:100098. https://doi.org/10.1016/j.xfnr.2025.100098 - Suda K, Nakaoka H, Yoshihara K, Ishiguro T, Tamura R, Mori Y, Yamawaki K, Adachi S, Takahashi T, Kase H, Tanaka K, Yamamoto T, Motoyama T, Inoue I, Enomoto T. Clonal Expansion and Diversification of Cancer-Associated Mutations in Endometriosis and Normal Endometrium. Cell Reports. 2018;24:1777-1789. https://doi.org/10.1016/j.celrep.2018.07.037 - Matsuzaki S, Canis M, Vaurs-Barrière C, Pouly JL, Boespflug-Tanguy O, Penault-Llorca F, Dechelotte P, Dastugue B, Okamura K, Mage G. DNA microarray analysis of gene expression profiles in deep endometriosis using laser capture microdissection. Molecular Human Reproduction. 2004;10:719-728. https://doi.org/10.1093/molehr/gah097 - Yan J, Zhou L, Liu M, Zhu H, Zhang X, Cai E, Xu X, Chen T, Cheng H, Liu J, Wang S, Dai L, Chang X, Tang F. Single-cell analysis reveals insights into epithelial abnormalities in ovarian endometriosis. Cell Reports. 2024;43:113716. https://doi.org/10.1016/j.celrep.2024.113716 - Matsuzaki S, Canis M, Vaurs-Barrière C, Boespflug-Tanguy O, Dastugue B, Mage G. DNA microarray analysis of gene expression in eutopic endometrium from patients with deep endometriosis using laser capture microdissection. Fertility and Sterility. 2005;84:1180-1190. https://doi.org/10.1016/j.fertnstert.2005.04.041 - Matsuzaki S, Canis M, Mage G. Use of laser capture microdissection in studying hormone-dependent diseases: endometriosis. Methods in Molecular Biology. 2009;590:295-306. https://doi.org/10.1007/978-1-60327-378-7_19 - da Costa K de A, Malvezzi H, Viana BG, Filippi RZ, Neme RM, Aloia TPA, Podgaec S, Piccinato CA. Validation of Laser Capture Microdissection Protocol in Endometriosis Studies. Medicina (Kaunas, Lithuania). 2019;55:520. https://doi.org/10.3390/medicina55090520 - Emmert-Buck MR, Bonner RF, Smith PD, Chuaqui RF, Zhuang Z, Goldstein SR, et al. Laser capture microdissection. Science. 1996;274:998-1001. https://doi.org/10.1126/science.274.5289.998 - Microdissection to microarrays: a complete solution for gene expression profiling of precious tissue samples. Application Note. 2016. Applied Biosystems, Thermo Fisher Scientific Inc. Doc. No. CO019851 0116. Accessed April 06,2026. https://www.thermofisher.com/lcm - Richter M, Piwocka O, Musielak M, Piotrowski I, Suchorska WM, Trzeciak T. From Donor to the Lab: A Fascinating Journey of Primary Cell Lines. Frontiers in Cell and Developmental Biology. 2021;9:711381. https://doi.org/10.3389/fcell.2021.711381 - Piwocka O, Musielak M, Ampuła K, Piotrowski I, Adamczyk B, Fundowicz M, Suchorska WM, Malicki J. Navigating challenges: optimising methods for primary cell culture isolation. Cancer Cell International. 2024;24:28. https://doi.org/10.1186/s12935-023-03190-4 - Zubeldia-Plazaola A, Ametller E, Mancino M, Prats de Puig M, López-Plana A, Guzman F, Vinyals L, Pastor-Arroyo EM, Almendro V, Fuster G, Gascón P. Comparison of methods for the isolation of human breast epithelial and myoepithelial cells. Frontiers in Cell and Developmental Biology. 2015;3:32. https://doi.org/10.3389/fcell.2015.00032 - Seelan RS, Warner DR, Mukhopadhyay PM, Andres SA, Smolenkova IA, Wittliff JL, Michele Pisano M, Greene RM. Epigenetic analysis of laser capture microdissected fetal epithelia. Analytical Biochemistry. 2013;442:68-74. https://doi.org/10.1016/j.ab.2013.07.029 - Braakman RB, Luider TM, Martens JW, Foekens JA, Umar A. Laser capture microdissection applications in breast cancer proteomics. Methods in Molecular Biology. 2011;755:143-154. https://doi.org/10.1007/978-1-61779-163-5_11 - Podgorny OV, Lazarev VN. Laser microdissection: A promising tool for exploring microorganisms and their interactions with hosts. Journal of Microbiological Methods. 2017;138:82-92. https://doi.org/10.1016/j.mimet.2016.01.001 - Chang CC, Chong HT, Tashiro A. Laser Capture Microdissection of Single Neurons with Morphological Visualization Using Fluorescent Proteins Fused to Transmembrane Proteins. eNeuro. 2021;8(5):ENEURO.0275-20.2021. https://doi.org/10.1523/ENEURO.0275-20.2021 - Satoskar AA, Shapiro JP, Bott CN, Song H, Nadasdy GM, Brodsky SV, Hebert LA, Birmingham DJ, Nadasdy T, Freitas MA, Rovin BH. Characterization of glomerular diseases using proteomic analysis of laser capture microdissected glomeruli. Modern Pathology. 2012;25:709-721. https://doi.org/10.1038/modpathol.2011.205 - Zwiers P, Querin V, Kiers J, Mecking M, Hackl M, Molema G, Bajema I. Laser microdissection combined with RNAseq reveals glomerulus-specific transcriptome profiles to discriminate class IV from class V lupus nephritis. Nephrology Dialysis Transplantation. 2025;40:gfaf116.1127. https://doi.org/10.1093/ndt/gfaf116.1127 - Navrazhina K, Garcet S, Williams SC, Gulati N, Kiecker F, Frew JW, Mitsui H, Krueger JG. Laser capture microdissection provides a novel molecular profile of human primary cutaneous melanoma. Pigment Cell and Melanoma Research. 2024;37:81-89. https://doi.org/10.1111/pcmr.13121 - Adamyan L, Aznaurova Y, Stepanian A, Nikitin D, Garazha A, Suntsova M, Sorokin M, Buzdin A. Gene Expression Signature of Endometrial Samples from Women with and without Endometriosis. Journal of Minimally Invasive Gynecology. 2021;28:1774-1785. https://doi.org/10.1016/j.jmig.2021.03.011 - Matsuzaki S, Canis M, Pouly J-L, Botchorishvili R, Déchelotte PJ, Mage G. Differential expression of genes in eutopic and ectopic endometrium from patients with ovarian endometriosis. Fertility and Sterility. 2006;86:548-553. https://doi.org/10.1016/j.fertnstert.2006.02.093 - Matsuzaki S, Canis M, Pouly JL, Dechelotte P, Okamura K, Mage G. The macrophage stimulating protein/RON system: a potential novel target for prevention and treatment of endometriosis. Molecular Human Reproduction. 2005;11:345-349. https://doi.org/10.1093/molehr/gah162 - Noë M, Ayhan A, Wang T, Shih I. Independent development of endometrial epithelium and stroma within the same endometriosis. The Journal of Pathology. 2018;245:265-269. https://doi.org/10.1002/path.5082 - Suda K, Nakaoka H, Yoshihara K, Ishiguro T, Adachi S, Kase H, Motoyama T, Inoue I, Enomoto T. Different mutation profiles between epithelium and stroma in endometriosis and normal endometrium. Human Reproduction. 2019;34:1899-1905. https://doi.org/10.1093/humrep/dez155 - Koppolu A, Maksym RB, Paskal W, Machnicki M, Rak B, Pępek M, Garbicz F, Pełka K, Kuśmierczyk Z, Jacko J, Rydzanicz M, Banach-Orłowska M, Stokłosa T, Płoski R, Malejczyk J, Włodarski PK. Epithelial Cells of Deep Infiltrating Endometriosis Harbor Mutations in Cancer Driver Genes. Cells. 2021;10:749. https://doi.org/10.3390/cells10040749 - Yachida N, Yoshihara K, Suda K, Nakaoka H, Ueda H, Sugino K, Yamaguchi M, Mori Y, Yamawaki K, Tamura R, Ishiguro T, Kase H, Motoyama T, Enomoto T. Biological significance of KRAS mutant allele expression in ovarian endometriosis. Cancer Science. 2021;112:2020-2032. https://doi.org/10.1111/cas.14871 - Li L, Facadio Antero M, Zhang M, et al. Mutation- and methylation-profiles of ectopic and eutopic endometrial epithelium. Journal of Pathology. 2021;255:387-398. https://doi.org/10.1002/path.5778 - Marshall A, Kommoss KF, Ortmann H, Kirchner M, Jauckus J, Sinn P, Strowitzki T, Germeyer A. Comparing gene expression in deep infiltrating endometriosis with adenomyosis uteri: evidence for dysregulation of oncogene pathways. Reproductive Biology and Endocrinology. 2023;21:33. https://doi.org/10.1186/s12958-023-01083-9 - Guo SW. Cancer-associated mutations in endometriosis: shedding light on the pathogenesis and pathophysiology. Human Reproduction Update. 2020;26:423-249. https://doi.org/10.1093/humupd/dmz047 - Mitchell D, Hunt AL, Conrads KA, Hood BL, Makohon-Moore SC, Rojas C, Maxwell GL, Bateman NW, Conrads TP. Industrialized, Artificial Intelligence-guided Laser Microdissection for Microscaled Proteomic Analysis of the Tumor Microenvironment. Journal of Visualized Experiments. 2022;(184):10.3791/64171. https://doi.org/10.3791/64171 - Choudhary RK, McFadden TB, Shangraw EM, Zhao F-Q. Optimized protocol to preserve RNA integrity for laser capture microdissection of bovine mammary epithelial cells. JDS Communications. 2025;6:624-627. https://doi.org/10.3168/jdsc.2024-0737 - Callens E, Syryn H, Deserranno K, Cattebeke G, Couckuyt H, Van De Velde J, Berrevoet D, Verplancke A, Deforce D, De Spiegelaere W, Van de Vijver K, Cools M, Van Nieuwerburgh F. A laser capture microdissection-based method for high-sensitivity transcriptomics from archived FFPE tissue slides with single-cell resolution using LCM-FFPEseq. bioRxiv. 2025.08.13.669096. https://doi.org/10.2139/ssrn.5503825 - Yan H, Shi J, Dai Y, Li X, Wu Y, Zhang J, Gu Z, Zhang C, Leng J. Technique integration of single-cell RNA sequencing with spatially resolved transcriptomics in the tumor microenvironment. Cancer Cell International. 2022;22:155. https://doi.org/10.1186/s12935-022-02580-4 - Göcz B, Rumpler É, Szentkirályi-Tóth S, Skrapits K, Takács S, Sárvári M, Farkas I, Póliska S, Hrabovszky E. Laser-capture microdissection for spatial transcriptomics of immunohistochemically detected neurons. The Journal of Biological Chemistry. 2025;301:108150. https://doi.org/10.1016/j.jbc.2024.108150 - Luo Y, Zhao C, Chen F. Multiomics Research: Principles and Challenges in Integrated Analysis. Biodesign Research. 2024;6:0059. https://doi.org/10.34133/bdr.0059 - Baião AR, Cai Z, Poulos RC, Robinson PJ, Reddel RR, Zhong Q, Vinga S, Gonçalves E. A technical review of multi-omics data integration methods: from classical statistical to deep generative approaches. Briefings in Bioinformatics. 2025;26:bbaf355. https://doi.org/10.1093/bib/bbaf355 - Subramanian I, Verma S, Kumar S, Jere A, Anamika K. Multi-omics Data Integration, Interpretation, and Its Application. Bioinformatics and Biology Insights. 2020;14:1177932219899051. https://doi.org/10.1177/1177932219899051 - Adamyan L, Pivazyan L, Zarova E, Avetisyan J, Laevskaya A, Sarkisova A, Stepanian A. Metabolomic biomarkers of endometriosis: A systematic review. Journal of Endometriosis and Uterine Disorders. 2024;7:100077. https://doi.org/10.1016/j.jeud.2024.100077 - Hernández A, Fernández-Medina O, Araiz PA, Terrón V, Martínez TR, Carrasco AL, Rodrigo MLJ, López-Collazo E, Del Fresno C, Spagnolo E. Identification of a circulating immunological signature as a liquid biopsy approach for the diagnosis of endometriosis. Scientific Reports. 2026;16:6052. https://doi.org/10.1038/s41598-026-36464-8 Подтверждение e-mail На [email protected] отправлено письмо со ссылкой для подтверждения e-mail. Перейдите по ссылке из письма, чтобы завершить регистрацию на сайте. Подтверждение e-mail Мы используем файлы cооkies для улучшения работы сайта. Оставаясь на нашем сайте, вы соглашаетесь с условиями использования файлов cооkies. Чтобы ознакомиться с нашими Положениями о конфиденциальности и об использовании файлов cookie, нажмите здесь.

Text is read by the "Ask this paper" AI Q&A widget below. Extraction quality varies by source — PMC NXML preserves structure cleanly, OA-HTML may include some navigation residue, and OA-PDF can have broken hyphenation. The publisher copy (via DOI) is the canonical version.

My notes (saved in your browser only)

⚙ Ask this paper AI returns verbatim quotes from the full text · source: oa-doi-fallback ⓘ

Answers must be backed by verbatim quotes from this paper's full text. Hallucinated quotes are dropped automatically; if no verbatim passage answers the question, we say so. How this works

Citation neighborhood

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

References (51)

Source provenance

openalex
last seen: 2026-08-09T06:04:05.141522+00:00
License: CC0 · commercial use OK